CTRC Safety Training March 10, 2014

1. CTRC Safety TrainingMarch 10, 2014

2. Provide Required Annual Safety Training for Personnel at the CTRC Purpose

3. CTRC Director- Dr Timothy Murphy • CTRC Manager- Dr. Richard Karalus • UB Biosafety Officer- David Pawlowski, Ph.D • UB Radiation Safety Officer- Jeff Slawson • Environmental Programs Manager- Brian Foti • Chemical Hygiene Officer, Hazardous Materials Manager- Anthony Oswald • Environmental Health and Safety Services 829-3301 • Employer (PI) responsibilities • Employee responsibilities • PIs bear full responsibility for safety in their laboratories Personnel and Responsibilities

4. Fire Safety

5. Fire Safety and Prevention • During a fire • Turn off oxygen, gas, andelectrical equipment in the affected area • USE THE STAIRS – NEVER USE THE ELEVATORS • Know primary and secondary evacuation routes

6. Fire Safety and Prevention • Bunsen Burners/open flame devices should be used only when necessary and should always be attended. • Consider bacticineratorsand micro burners as alternatives to Bunsen Burners. • Biosafety Cabinets - open flames are NOT recommended. They can damage the HEPA filter and cause a fire.

7. Fire Safety and Prevention • Extension cords are not permitted for permanent applications • Space heaters must be equipped with tip over shutoff devices • Daisy Chained power strips are not permitted • Maintain a minimum of 18" between boxes and ceiling

8. Fire alarm pull stations • Each stairwell entrance • CRC • Fire extinguishers • Emergency showers • Emergency eye wash stations • AEDs near SW corner of each floor • First aid kits Know locations of safety equipment Safety Equipment

9. Fire Safety • Fire alarm pull stations are located by each stair well • Fire alarms will include both strobe and audio alarms • CTRC alarms only alarm floor involved and the adjacent floors • Only alarming floors are required to evacuate unless otherwise advised

10. Fire Extinguishers • Fire Extinguishers: • Located in most laboratories • Do not obstruct or concealfire extinguishers • Located in hallwaysthroughout CTRC • Know where your nearestfire extinguisher is

11. Fire Extinguishers • When using remember P.A.S.S. P = Pull the pin A = Aim at base of fire about 8-10ft away S = Squeeze the trigger S = Sweep side to side • “How to Use” instructions can be found on thefire extinguisher label • USE ONLY IF FIRE IS SMALL AND IFYOU HAVE HAD TRAINING ON ITS USE

12. Fire:Remember R.A.C.E. R = Rescue Rescue people in the immediate area Announce the fire verballyActivate the alarm A = Announce C = Confine Confine fire by Closing doors E = Evacuate Evacuate the floor, Extinguish if a small fire

13. Evacuation-Fifth Floor

14. Evacuation-Sixth Floor

15. Evacuation-Seventh Floor

16. Evacuation-Eighth Floor

17. Section #3 Ground Floor Evacuation

18. Sub-basement Level Evacuation

19. General Laboratory Safety

20. BSL-1 • Organisms that do not normally cause human infections • BSL-2 • Organisms that cause human infections of low morbidity/mortality • Potential for aerosol transmission • BSL-3 • Organisms that cause human infections of high morbidity/mortality • Aerosol transmission • BSL-4 • Organisms of extremely high morbidity/mortality for which there are no treatments • Aerosol or unknown transmission Biosafety levels

21. General laboratory rules • Eating, drinking, smoking or the use of other tobacco products or cosmetics is strictly prohibited • The application or removal of contact lenses is forbidden • Storage of these items in the laboratory is prohibited • Mouth pipetting is strictly prohibited • Open toed shoes are not permitted • Minimize aerosols • Wash Hands General Safety

22. Should never serve as primary protection • Appropriate for risk • Minimum • Disposable surgical gloves • Nitrile (recommended) • Latex • Eye protection • Splash • UV/other radiation • Lab Coat • Respirators (may require fit testing and medical clearance- contact EH&S) • Particulate (N95, PAPR, P-100) • Chemical cartridge (activated charcoal, chlorine, etc) • Hearing Personal Protective Equipment

23. PPE Rules to Remember Always check PPE for defects or tears before using If PPE becomes torn or defective remove and replace Remove PPE before leaving a contaminated area Contaminated PPE should be removed and disposed of in biohazard containers Do not reuse disposable equipment

24. HVAC negative pressure • Fume hoods • Use with volatile chemicals or non-infectious substances that pose an aerosol risk • Toxic powders Note: The use of biological agents in a fume hood is prohibited. • Use Biosafety cabinets with BSL-2 (or higher) agents where an aerosol hazard exists • Electrical Protection, • GFI • Sound cabinets • sonicators Engineering Controls (Facilities and Equipment that enhance safety)

25. General Operation • Types • Class I- no longer used • Class II type A/B3- 70% recirculation, 30% exhaust into room (type A); or thimble connected to building HVAC- negative pressure plenum (type B3) • Class II type B1- Cabinet air is 40% recirculated, hard ducted to HVAC, can be used with minute amounts of chemicals. • Class II type B2- 100% exhaust can be used with small amounts of chemicals, plenum is totally under negative pressure. • Class III- glovebox • Must be certified at least annually- PI responsibility Biosafety Cabinets

26. Do not store chemicals or equipment in fume hoods Use appropriate PPE Use with sash in proper position Check for properairflow before using(e.g., “tissue on sash alarm”) Report any diminishedairflow to Building Manager (888-4730) Fume Hoods

27. Lab Specific Safety Training • The Lab supervisor/PI is required to provide lab specific safety training to staff working in their in their lab(s) • Laboratory Specific training should supplement general training on laboratory specific hazards and safety procedures • Additional specific training (radiation safety, animal handling, etc.) may also be required NOTE: The PI is responsible for safety in his/her laboratory

28. OSHA BloodBorne Pathogen Standard

29. Laboratory accidents Sharps Spills [Animal exposure (LAF Occupational Exposure Medical Plan)]* Handling of human (animal*) samples Handling of any waste products First aid administration Post-accident cleanup Janitorial or maintenance work Your Exposure Potential *Animal tissues and fluids are not included in the official OSHA BloodBorne Pathogen Standard, but pose similar risks and are thus included in this discussion

30. Hepatitis B(HBV) • Hepatitis C(HCV) • Human Immunodeficiency Virus (HIV) Common Bloodborne Pathogens

31. Human (and Animal*) • Blood • Skin and tissue • Cell cultures • Saliva • Vomit • Urine • Semen and vaginal secretions • Any bodily fluid or substance Potentially Infectious Substances

32. Treat all blood and bodily fluids and samples as if they are contaminated Proper cleanup and decontamination Dispose of contaminated materials in the proper biohazard containers Use of proper PPE Universal Precautions

33. Wash hands immediately after removing PPE and before leaving laboratory Use a soft antibacterial soap Do not use bleach A hand sanitizer can be used, but wash with soap and water as soon as possible. Hand Washing

34. Vaccinations • Hepatitis B • Animal Handling* • (LAF Occupational Exposure Medical Plan) • Exotic agents Medical Program

35. Exposure Incident OSHA requirement: Each lab should have a written, lab specific Exposure Control Plan (template available at EH&S website) An exposure is a specific incident of contact with potentially infectious bodily fluid If there are no infiltrations of mucous membranes or open skin surfaces, it is not considered an occupational exposure Report all accidents involving blood or bodily fluids to your superior and UB EH&S Post-exposure medical evaluations must be offered (personal physician or clinic)

36. Confidential medical evaluation Document route of exposure Identify source individual Test source individual’s blood (with individual’s consent) Provide results to exposed employee File C2 workers accident form with NYS Workers Compensation Board (1-866-396-8314) Post-Exposure Evaluation

37. Strongly endorsed by medical communities Offered to all employees working with bloodborne hazards- must be documented Provided at no cost to employees Declination form Hepatitis B Vaccination

38. Decontamination Wear appropriate PPE When decontaminating surfaces use appropriate disinfectant Cover contaminated area with disinfectant, allow appropriate contact time, and wipe up Dispose of all wipes in biohazard containers

39. Know the proper decontaminant and proper usage for each agent Common Examples: • Bleach (sodium hypochlorite) • 1:10 dilution 5.25% household bleach (5,000 ppm free chlorine) • 30 minute exposure time • 2 week shelf life (diluted) • 6 month shelf life (undiluted) • Corrosive to metal • Ethanol • 70% • Rapidly bactericidal • Noncorrosive • Flammable • Not sporicidal • Unable to kill hydrophilic viruses • May increase latex permeability to viruses Chemical Decontamination

40. Operation • 121o C for a minimum of 20 minutes. • Bags should be no more than 2/3 full • Bags should not be completely sealed during autoclaving • Bags should be placed in a container capable of containing any contents that may leak from them • Validation • An indicator must be present in each load (autoclave tape, steam strip, spore test) Autoclave Use

41. Laboratory Door Posting • Required on each research lab door • Quick reference in caseof an emergency or an issue concerning safety • Fillable order form available on EH&S site: http://www.facilities-buffalo.org/Departments/ehs/EHSForms • Reviewed and updated annually or whenever a significant change takes place

42. Lab Security • Report any suspicious individuals immediately to Kaleida Security (859-2196) • Report any lost, missing, or stolen hazardous materials (biologicals, chemicals, radioactive materials, etc.) to EH&S (829-3301) • Report any lost, stolen, or found keys, or any failures of the security doors immediately to Kaleida Security and the CTRC Manager (888-4730)

43. Lab Security Security is only as strong as the occupants wish it to be • No tailgating(one swipe card = one person in) • Doors should not be propped open • If someone looks out of place, ask if they need assistance • Do not leave valuables in the open • Lock doors when rooms are vacant

44. Chemical Safety

45. Regulatory Agencies forHazardous Chemicals • Occupational Safety and Health Administration (OSHA) [Workplace] • New York State Department of Labor (DOL) [private firms] • Public Employee Safety and Health Bureau (PESH)- NYS employees • National Fire Protection Association (NFPA) [Fire Protection and Storage]

46. Agencies that RegulateHazardous Waste Hazardous waste generators must comply with regulations enforced by these agencies: • Environmental Protection Agency (EPA) • New York State Department of Environmental Conservation (NYSDEC) • Department of Transportation (DOT)

47. Flammable Chemicals • Flash point <100°F (Combustible - Flash Point >100-200°F) • Fire/Explosion Hazard • Keep Sparks and Flames Away • Examples • Acetone, Ethanol, Methanol

48. Reactive Chemicals • Release Large Amounts of Energy • React Violently with Water or Air • React with Other Chemicals to Produce Toxic Gases • Rapid Pressure Build-up/Explosion Potential • Unstable/Readily Undergoes Change • Examples: • Calcium Hydride, Sodium Metal, and Organic Peroxides

49. Corrosive Chemicals • Acids or Alkalis (Bases) • Destructive to Tissue • Generates Heat During Reactions • Examples: • Hydrochloric Acid, Potassium Hydroxide, Hydrofluoric acid

50. Sensitizer Chemicals • Allergic Reaction • Repeated exposure may worsen reaction • Individuals React Differently! • Severity Depends on Sensitivity, Potency, Concentration, and Duration • Examples: • Poison Ivy, Chromic Acid, Nickel