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1. Application of Solid Phase Microextraction -Gas Chromatography - Mass Spectrometry in Flavor Research David B. Min
Department of Food Science and Technology
The Ohio State University
Columbus, Ohio USA
2. Isolation, Separation and Identification of Flavor Compounds of Edible Oil
3. Solid Phase Microextraction-Gas Chromatography-Mass Spectrometry
4. Principle of Solid Phase Micro Extraction
5. Solid Phase Micro Extraction for Headspace Flavor Compounds Isolation
6. SPME Principles for Headspace Flavor Compounds Isolation
7. Fibers on the Volatile Compounds Extraction
8. Volatile Compounds Isolation by SPME
9. Gas Chromatography-Mass Spectrometry
10. Gas Liquid Chromatography gas system
inlet
column
detector
data system
11. Principle of Gas Liquid Chromatography Partition of compounds between gas mobile phase and liquid stationary phase.
The compound which has high affinity to the liquid stationary phase takes longer to pass through the column than the compound with less affinity to the stationary phase of column.
12. Schematic Diagram of Gas Chromatograph
13. Liquid Stationary Phases
14. Flame Ionization Detector
15. Diagram of Flame Ionization Detector
16. Volatile Compounds Isolation by SPME
17. Effect of G.C.Injection Temperature on Corn Oil Volatile Compounds Isolated by SPME
18. Chromatograms of Corn Oil Volatile Compounds Isolated by SPME
19. Effect of Isolation Temperature on Corn Oil Volatile Compounds by SPME
20. Isolation Time Effect on Corn Oil Volatile Compounds by SPME
21. Chromatograms of Soybean Oil and Corn Oil
22. Improving the Sensitivity of Solid Phase Microextraction
23. Gas Chromatogram of Corn Oil Volatile Compounds Isolated by Solid Phase Microextraction
24. Gas Chromatography-Mass Spectrometry
25. Principle of Mass Spectrometry A mass spectrometer bombards a compound with an electron
beam and quantitatively records the result as a spectrum of
positive ion fragments.
A mass spectrum is a presentation of the masses of the
positively charged fragments vs. their relative concentration.
26. Gas Chromatogram of Corn Oil Volatile Compounds Isolated by Solid Phase Microextraction
27. Quadruple Mass Spectrometer
28. Fragmentations of 2-Methylpentane by Electron Bombardment
29. Quadruple Analyzer for the Separation of Positive Ion Fragments
30. Mass Spectrum of 2-Methylpentane
31. Gas Chromatogram of Corn Oil Volatile Compounds Isolated by Solid Phase Microextraction
32. Corn Oil Volatile Compounds by SPME-GC-MS
33. SPME - GC - MS