What are the symptoms observed in Cystic Fibrosis Patients?

1. What are the symptoms observed in Cystic Fibrosis Patients? 1. 2. 3. 4. Thick mucus in airways and lungs and breathing problems Chronic lung infections Digestive problems that lead to poor growth Increased salty sweat X X X X

2. Genotype Nomenclature What is the carrier status? 1. 2. 3. CC Non-carrier of CF Cc Carrier of CF cc CF patient X X X X

3. What Do the letters in ELSI stand for? 1. 2. 3. 4. Ethical E Legal L Social S I Implications X X X X

4. What do you know about CFTR 1. 2. 3. 4. 5. It stands for cystic fibrosis transmembrane conductance regulator It functions as a chloride ion channel It is localized to the plasma membrane Cystic fibrosis is caused by a mutation in the CFTR gene There are over 400 different mutations known for CFTR gene X X X X

5. Mucus, Mucus, Mucus 1. 2. 3. 4. 5. What does mucus do? Traps dust, dirt, germs in the lining of your nose, trachea and lungs What are cilia? Tiny hairs that push mucus out of your lungs and into your throat Where is mucus made? Mucus glands What is the mucociliary escalator? The system of mucus and cilia that rids our lungs of bacteria What is different about the mucus of people with CF? There are over 400 different mutations known for CFTR gene X X X X

6. What about water inside our cells? 1. 2. 3. 4. If the concentration of chloride ions is higher inside the cell? Water is drawn into our cells Channels How does it cross the cell membrane? Water is drawn out of the cell If the concentration of chloride ions is higher outside the cell? What is the process of water following the concentration gradient called? Osmosis X X X X

7. Making CFTR Protein 1. 2. 3. 4. 5. Where is the CFTR gene found On the chromosome Where does transcription occur? In the cell nucleus Where does translation occur? Cell cytoplasm What is the CFTR gene transcribed into? CFTR messenger RNA What is the CFTR mRNA translated into? CTFR protein X X X X