1 / 41

Fertilization

Fertilization. Animal Science 434 John J. Parrish. Capacitation. 1951 Austin - rat Chang - rabbit Fertilization does not occur until several hours after sperm are placed in the uterus or oviduct. Chang Experiment - 1951. Ovulation. After. Before. Percent Fertilization. Capacitation.

goldy
Download Presentation

Fertilization

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Fertilization Animal Science 434 John J. Parrish

  2. Capacitation • 1951 • Austin - rat • Chang - rabbit • Fertilization does not occur until several hours after sperm are placed in the uterus or oviduct

  3. Chang Experiment - 1951 Ovulation After Before Percent Fertilization

  4. Capacitation The changes within sperm that confer upon the sperm the ability to acrosome react in response to the appropriate stimulus. • Zona Pellucida • Cumulus Cells • Follicular Fluid

  5. Epididymal Ejaculated Capacitated Seminal Plasma and/or Epididymal Secretions Female Tract + + Effect of Capacitation

  6. DF (BSP in Bovine) Plasma Membrane Decapacitation Factor – Stabilizes plasma membrane and inhibits a premature acrosome reaction

  7. BSP Cholesterol Acceptor Heparin Plasma Membrane

  8. BSP Cholesterol Acceptor Heparin Zp binding clustering Raft Migration Plasma Membrane

  9. BSP HCO3- Cholesterol Acceptor H+ Heparin Zp binding clustering Raft Migration sAC (+) H+ HCO3- cAMP (+) (+) pHi PK-A (+) (-) PTK Ptyr-Ptase (+) (-) Protein Tyrosine Phosphorylation

  10. Extracellular Space Diffusion CO2 + H2O H2CO3 H+ +HCO3- Carbonic Anhydrase

  11. BSP HCO3- Cholesterol Acceptor H+ Heparin Zp binding clustering Raft Migration sAC (+) H+ HCO3- cAMP (+) (+) pHi PK-A (+) (-) PTK Ptyr-Ptase (+) (-) Protein Tyrosine Phosphorylation

  12. ATP ADP Ca2+ Ca2+ ADP ATP Ca2+ Acrosome

  13. BSP ATP ADP Ca2+ Ca2+ Cholesterol Acceptor Heparin Raft Migration ADP ATP Ca2+ Ca2+ Acrosome

  14. ATP ADP Ca2+ BSP HCO3- Cholesterol Acceptor H+ Ca2+ Heparin sAC (+) (+) H+ HCO3- Ca2+ cAMP (+) (+) pHi PK-A (+) Ca2+ (-) Acrosome PTK Ptyr-Ptase (+) (-) Protein Tyrosine Phosphorylation

  15. Capacitation • No change in sperm morphology • Motility change • hyperactivation cAMP Power output

  16. Fused Plasma Membrane and Outer Acrosomal Membrane The Acrosome Reaction Plasma Membrane Acrosin Hyaluronidase Acrosome Nucleus

  17. Modification of the Equitorial Region

  18. The Zona Pellucida ZP1 ZP2 ZP3

  19. Sperm Interaction with the Zona Attachment Zona Pellucida Binding ZP3 Acrosome Reaction Induction Acrosome Reaction

  20. ZP2 ZP2 ZP2 ZP2 Secondary Binding Zona Pellucida Zona Penetration Motility

  21. Zona Pellucida

  22. Sperm tract through zona pellucida

  23. 6-12 hr 18-24 hr

  24. MPF MPF MPF Maturation Promoting Factor(MPF) LH Surge Metaphase Promoting Factor Primary Oocyte GVBD Metaphase I GV-Intact PB-1 Ovulation MPF Metaphase II Secondary Oocyte

  25. Ca2+ Ca2+ Ca2+ Ca2+ Zona Pellucida Oocyte Perivitelline Space

  26. Ca2+ Ca2+ Ca2+ Ca2+ Zona Pellucida Oocyte Perivitelline Space

  27. Ca2+ Ca2+ Ca2+ Ca2+ Zona Pellucida Oocyte Perivitelline Space

  28. Ca2+ Ca2+ Ca2+ Ca2+ Zona Pellucida Oocyte Perivitelline Space

  29. Ca2+ Ca2+ Ca2+ Ca2+ Zona Pellucida Oocyte Perivitelline Space

  30. Calcium Transients During Bovine Fertilization

  31. Initiation of Calcium Pulse by Soluble Sperm Component DAG Oocyte PIP2 PLCzeta IP3 Ca2+ Ca2+ Endoplasmic Reticulum

  32. Pronuclei Ca2+ AII X TII MII PBII Zona Pellucida Oocyte MPF Perivitelline Space

  33. Decondensation Pronuclei Pronuclei Ca2+ AII X TII MPF MII PBII Zona Pellucida Oocyte Zygote Perivitelline Space

  34. Ca2+ Cortical Granules Zona Pellucida Oocyte Zona Block to Polyspermy Perivitelline Space

  35. Ca2+ Zona Pellucida Oocyte Zona Block to Polyspermy Cortical Granules Perivitelline Space

  36. Blocks to Polyspermy Species 1. Mammals 3. InvertebratesAmphibiansFishRabbits 4. AmphibiansAvians 1 2 3 4

  37. Gamete Aging • Oocyte life span approx. 6 – 12 hours after ovulation • Sperm life span approx. 24 – 48 hours after ejaculation

More Related