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STEM Electrophoresis with Food Dyes. Bio-Rad Biotechnology Explorer IDEA and STEM Kits. Separation technology: How do you separate two or more compounds from each other?. NH 3 +. -. -. -. -. -. -. -. -. -. O. HO CH 2 C CH 2 OH. CH 3 C. -. O -. CH 2 OH. +. +.
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STEM Electrophoresis with Food Dyes Bio-Rad Biotechnology Explorer IDEA and STEM Kits
Separation technology: How do you separate two or more compounds from each other?
NH3+ - - - - - - - - - O HO CH2 C CH2 OH CH3 C - O- CH2 OH + + + + + + + + + Apply an electrical charge + Electricity can be used to separate molecules by charge Acetate ions Tris ions
Electrophoresis separates molecules by CHARGE and SIZE Electrophoresis means “to carry with electricity” Molecular sieve Electricity
Food dyes – What have you recently eaten?… Did you eat any cheddar cheese? Was it orange? Starbucks Strawberry Frapuccino?
FD&C Dyes and Natural dyes FD&C Synthetic Dyes FD&C Red 40 or FD&C Red 40 Lake FD&C Yellow 6 or FD&C Yellow 6 Lake FD&C Yellow 5 or FD&C Yellow 5 Lake FD&C Blue 1 or FD&C Blue 1 Lake FD&C Blue 2 or FD&C Blue 2 Lake FD&C Red 3 or FD&C Red 3 Lake FD&C Green 3 or FD&C Green 3 Lake Natural Dyes Beetroot red or betanin (from beets) Curcumin (from tumeric) Caramel coloring (from sugar) Annatto (seeds of achiote trees) Carminic acid, carmine, or cochineal (from ground up beetle abdomens) Lycopene (from tomatoes, watermelons, papayas, and red carrots)
Food dyes have an intrinsic SIZE and CHARGE and thus can be separated using Electrophoresis
So how do we design an electrophoresis chamber to separate food dyes? Dye Electrophoresis Commercial versus built box comparisons
Building and running your electrophoresis system to separate the dyes
Building and running your electrophoresis system to separate the dyes
Building and running your electrophoresis system to separate the dyes
Building and running your electrophoresis system to separate the dyes
Building and running your electrophoresis system to separate the dyes
Extensions Copper electrode • Electrochemistry study • pH changes at the cathode and anode • Effect of material choice for electrode • Optimization of STEM box system • Electrode material choice and thickness • Different materials for gel thickness • Impact of TAE Concentration and Volume • Gel percentages Gelatin matrix