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STEM Electrophoresis with Food Dyes

STEM Electrophoresis with Food Dyes. Bio-Rad Biotechnology Explorer IDEA and STEM Kits. Separation technology: How do you separate two or more compounds from each other?. NH 3 +. -. -. -. -. -. -. -. -. -. O. HO CH 2 C CH 2 OH. CH 3 C. -. O -. CH 2 OH. +. +.

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STEM Electrophoresis with Food Dyes

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  1. STEM Electrophoresis with Food Dyes Bio-Rad Biotechnology Explorer IDEA and STEM Kits

  2. Separation technology: How do you separate two or more compounds from each other?

  3. NH3+ - - - - - - - - - O HO CH2 C CH2 OH CH3 C - O- CH2 OH + + + + + + + + + Apply an electrical charge + Electricity can be used to separate molecules by charge Acetate ions Tris ions

  4. Sieves can be used to separate materials by their size

  5. Electrophoresis separates molecules by CHARGE and SIZE Electrophoresis means “to carry with electricity” Molecular sieve Electricity

  6. Food dyes – What have you recently eaten?… Did you eat any cheddar cheese? Was it orange? Starbucks Strawberry Frapuccino?

  7. FD&C Dyes and Natural dyes FD&C Synthetic Dyes FD&C Red 40 or FD&C Red 40 Lake FD&C Yellow 6 or FD&C Yellow 6 Lake FD&C Yellow 5 or FD&C Yellow 5 Lake FD&C Blue 1 or FD&C Blue 1 Lake FD&C Blue 2 or FD&C Blue 2 Lake FD&C Red 3 or FD&C Red 3 Lake FD&C Green 3 or FD&C Green 3 Lake Natural Dyes Beetroot red or betanin (from beets) Curcumin (from tumeric) Caramel coloring (from sugar) Annatto (seeds of achiote trees) Carminic acid, carmine, or cochineal (from ground up beetle abdomens) Lycopene (from tomatoes, watermelons, papayas, and red carrots)

  8. Food dyes in the news

  9. Food dyes have an intrinsic SIZE and CHARGE and thus can be separated using Electrophoresis

  10. So how do we design an electrophoresis chamber to separate food dyes? Dye Electrophoresis Commercial versus built box comparisons

  11. What are some of the design factors we want to think about?

  12. Building and running your electrophoresis system to separate the dyes

  13. Building and running your electrophoresis system to separate the dyes

  14. Building and running your electrophoresis system to separate the dyes

  15. Building and running your electrophoresis system to separate the dyes

  16. Building and running your electrophoresis system to separate the dyes

  17. Extensions Copper electrode • Electrochemistry study • pH changes at the cathode and anode • Effect of material choice for electrode • Optimization of STEM box system • Electrode material choice and thickness • Different materials for gel thickness • Impact of TAE Concentration and Volume • Gel percentages Gelatin matrix

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