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TRPGR Gramene: A Platform for Comparative Plant Genomics. Doreen Ware USDA ARS Cold Spring Harbor Laboratory Pankaj Jaiswal Oregon State University Joshua Stein Cold Spring Harbor Laboratory. www.gramene.org Workshop website http://outreach.gramene.org. Agenda.
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TRPGR Gramene: A Platform for Comparative Plant Genomics Doreen Ware USDA ARS Cold Spring Harbor Laboratory Pankaj Jaiswal Oregon State University Joshua Stein Cold Spring Harbor Laboratory www.gramene.org Workshop website http://outreach.gramene.org
Agenda • 7.30-7.40pm Gramene Database Workshop Introduction: Doreen Ware • 7.40-8.00pm Exploring the Plant Genomes (browse, search, upload personal data, analysis tools): Josh Stein • 8.00-8.15pm Phylogenetic analysis tools and targeted query tools using Biomart: Josh Stein • 8.15-8.35pm Exploring the Plant's Genetic Diversity: Doreen Ware • 8.35-8.50pm Comparative Pathways Networks and future directions: Pankaj Jaiswal • 8.50-9.00pm Q/A • Exhibit 9:00- 11:00pm Q/A
Gramene: A Platform for Comparative Plant Genomics Principle Investigators Doreen Ware (USDA ARS) Susan McCouch Edward Buckler (USDA ARS) Pankaj Jaiswal Biological Curation Genevieve DeClerck Palitha Dharmawardhana Vindhya Amarasinghe SushmaNaithani Charles Chen A. S. (Karthik) Karthikeyan Joshua Stein Bioinformatics Developers Terry Casstevens Justin Preece Marcela Monaco Will Spooner Jim Thomason Sharon Wei Ken Youens-Clark Aaron Chuah (past) Jon Zhang . Cold Spring Harbor Cornell University Oregon State University • Gramene is currently funded by NSF Grant 0703908: Plant Genome Research Resource • USDA, DOE, NSF (Previous funding 2001-2007 ) • Past PIs Lincoln Stein, Sam Cartinhour
“What are the functionally shared elements of plant genomes and how does diversity in these elements relate to agronomic traits?”
Polymorphism Candidate gene Gene models Transcripts Peptides Function Expression Pathway Orthologs Markers & phenotypes (Genes and QTL) Map-1 Map-2 Physical and/or Sequence map Comparative and Translational Genomics Genotype Forward Reverse Phenotype
A network of information Phenotype Analysis Genotype Assay Germplasm Marker Location Map Genome Pathway
What is Gramene? What do we do? • Community portal • Contribute to open standards (PO, TO, GO) • Create and contribute to open software (Ensembl, CMap, CPAN, Tassel) • Curators and stewards of genomes, diversity & pathway data • Active research using comparative resources
Academic and Industry users throughout US, Europe and Asia Sep 2009 – Sep 2010
www.gramene.org Collaborators Individual Researchers EBI/Ensembl Genomes MSU TAIR JGI MaizeGDB SCRI MIPS NASC SRI (Pathway Tools) OMAP/OGE GrainGenes Gene Ontology Plant Ontology Reactome BAR iPlant