1 / 15

Yasushi Sako, Akira Nagafuchi, Shoichiro Tsukita, Masatoshi Takeichi, and Akihiro Kusumi

Cytoplasmic Regulation of the Movement of E-Cadherin on the Free Cell Surface as Studied by Optical Tweezers and Single Particle Tracking: Corralling and Tethering by the Membrane Skeleton. Yasushi Sako, Akira Nagafuchi, Shoichiro Tsukita, Masatoshi Takeichi, and Akihiro Kusumi. J. Cell Biol.

gwyn
Download Presentation

Yasushi Sako, Akira Nagafuchi, Shoichiro Tsukita, Masatoshi Takeichi, and Akihiro Kusumi

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Cytoplasmic Regulation of the Movement of E-Cadherin on the Free Cell Surface as Studied by Optical Tweezers and Single Particle Tracking: Corralling and Tethering by the Membrane Skeleton Yasushi Sako, Akira Nagafuchi, Shoichiro Tsukita, Masatoshi Takeichi, and Akihiro Kusumi J. Cell Biol. Volume 140(5):1227-1240 March 2, 1998

  2. A tether model (A) and a fence model (B) proposed as mechanisms for the regulation of lateral movements of E-cadherin (Sako and Kusumi, 1995) Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  3. Structures of the wild-type E-cadherin and its artificial mutants used in this study Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  4. Indirect immunofluorescence staining of E-cadherin on the surface of cells transfected with E-cadherin cDNAs Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  5. Trajectories of particle–cadherin complexes dragged by OT Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  6. (A) The time course of a representative dragging experiment Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  7. Distributions of the escape distances Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  8. Distributions of the freely dragged distances Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  9. Distributions of the effective spring constant (kmsk) of the portions of the membrane skeleton/cytoskeleton that are involved in corralling or tethering E-cadherin molecules Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  10. kmsk (effective spring constant) plotted against the freely dragged distance (dfd) for individual particles Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  11. SPT trajectories of E-cadherins recorded for 16.7 s (500 video frames) Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  12. Distributions of Dmicro Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  13. Classification of trajectories into simple Brownian, confined, and directed movement modes in a time window of 5 s Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  14. Typical trajectories of Wild (A) and Fusion (B) classified as directed movement Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

  15. Models of the mechanisms for the regulation of the movement of E-cadherins in the plasma membrane Sako Y. et.al. J. Cell Biol. 1998:140:1227-1240

More Related