Background : Diabetes is a metabolic disorder of multiple etiologies

1. EFFECT OF CARVACROL AS AN ANTIDIABETIC AGENT ON STREPTOZOTOCIN INDUCED DIABETIC RATS.m.najmaHabeeb and Prakash R. Naik*Department of Zoology, University of Mysore, Mysore-06 Result: ` . The values are mean ±S.E. Superscripts (a,b,c) are obtained from Duncan’s post hoc test Background:Diabetes is a metabolic disorder of multiple etiologies characterized by chronic hyperglycemia with disturbance of carbohydrate, fat, and protein metabolism resulting from defects in insulin secretion, insulin action, or both. Diabetes is a global epidemic with an estimated worldwide prevalence of 246 million people in 2007 and forecasts to rise to 300 million by 2025 . Abstract: Natural antidiabetic agents are considered to be useful agents for the prevention of diseases, especially the most debilitating and often life-threatening disease like diabetic mellitus. Carvacrol treated animals showed significant change in the blood glucose and the body weight. The altered lipid profile was reversed towards normal TC, LDL,VLDL and TG, fall in HDL. • Conclusion: • There was a significant decrease in the blood glucose level in the Carvacrol treated when compare with that of the diabetic control. Carvacrol is present in the essential oil of Origanumvulgare (oregano), oil of thyme, oil obtained from pepperwort, and wild bergamot. • Interestingly there was a significant difference in the body weight of the treated animals as compare with that of the diabetic control and the normal group. There was a significant alteration in the enzyme activity in the treated animals compare to that of the diabetic animals. • The fasting insulin level also showed significant enhancement in insulin level when compared with that of the diabetic group. • HOMA-IR showed significant difference between the normal and the treated groups-50mg, 100mg and 150mg . Fig 1: body weight of different experimental groups. Fig 2: Serum glucose level of different experimental groups • Enzymes like Hexokinase and Glucose 6 phosphatase level significantly increased in the treated group compared with that of the diabetic, • While Glucose 6 phosphate dehydrogenase significantly decreased in the treated group compared with that of the diabetic group. • There was a significant change observed in the concentration of Glutathione S-transferase and Glutathione reductase when compared with the carvacrol treated and the diabetic groups. • High levels of triglycerides and total cholesterol which are typical of the diabetic condition. The carvacrol treatment significantly decreased the triglycerides levels and normalized the total cholesterol concentration. Fig 3. Comparison of lipid profile of different experimental groups. Fig 4. Comparison between serum urea and glycogen of different experimental groups. Objective: The present investigation was carried out to assess the effect of carvacrol on streptozotocin induced diabetic rats. • Hence the positive effect of carvacrol on diabetic animals suggests a possible role in improving glucose metabolism. • Reference: • Whiting DR., Guariguata L., Weil C., and Shaw J. IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Research and Clinical Practice, 2011;94:311–321. • Morel DW and Chisolm G M. Antioxidant treatment of diabetic rats inhibits lipoprotein oxidation and cytotoxicity.Journal of Lipid Research, 1989; 30(12): 1827–1834.  • Bunn HG., Gabby KH., Gallop PM. The glycosylation of hemoglobin: relevance to diabetes mellitus. Science;1978: 200, 21–27. Method:  . Group 1: Control group Group 2: Diabetic rats Group 3: Diabetic rats treated with glibenclimide 1 mg/kg body weight. Group 4: Diabetic rats treated with 50 mg carvacrol/kg body weight. Group 5: Diabetic rats treated with 100 mg carvacrol/kg body weight. Group 6: Diabetic rats treated with 150 mg carvacrol/kg body weight Fig 5 . Comparison of insulin level in different experimental groups. Fig 6. Comparison of HOMA-IR of different experimental groups. Acknowledgement :The first author acknowledges UGC-RFSMS for financial support. Oralintubation Streptozotocin injection Contact: : Prof. Prakash R. Naik Department of Studies in Zoology, Manasagangotri,University of Mysore, Mysore-06. Phone number: +91-9448352965 E-mail address : prakashrnaik@yahoo.co.in Fig 7 . Comparison of GST level in different experimental groups. Fig 8. Comparison of enzymes of different experimental groups.