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AP Bio Lab 1

AP Bio Lab 1. Water Potential. The combined effect of: Solute concentration Pressure …are incorporated into a single measurement called water potential Water moves from the area of HIGH water potential  to the area of LOW water potential when moving across a membrane.

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AP Bio Lab 1

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  1. AP Bio Lab 1

  2. Water Potential • The combined effect of: • Solute concentration • Pressure …are incorporated into a single measurement called water potential • Water moves from the area of HIGH water potential  to the area of LOW water potential when moving across a membrane

  3. *H20 moves from areas of high pressure to areas of low pressure*

  4. Solute Potential • Pure water has a solute potential of zero. Solute potential is NEVER positive. • Adding more solute is a negative experience; the solute potential always becomes negative or more negative Sample Problem • The molar concentration of a sugar solution in an open beaker has been determined to be 0.3M. Calculate the solute potential at 27 degrees. Round your answer to the nearest hundredth. • If the sugar solution was placed inside of dialysis tubing and put in a beaker of pure water (Ψ=1) what would happen? Why?

  5. Pressure Potential • Is the sum of all pressure on water • Get b/c of 2 things: • Turgor pressure- force created by the cell membrane pushing against the cell wall (must have water inside to push cell wall out) • You CAN’T know this unless given so assume that the pressure potential is zero unless otherwise told

  6. QUIZ TIME!!!!

  7. Lab Set Up • Changes to procedure: • Part B, bags are 15cm long NOT 30cm • Part B, add 10ml of each sucrose solution to each bag • You don’t know the molarities they are labeled (A-F). You must determine which solution goes to which molarity based on your collected data. • Put all dialysis bags and string in the same beaker for the weekend and cover! • No cork borer for potato cylinders so cut 4 relatively equally sized cubes for each solution  REMOVE ALL SKIN (set up scales first before getting so you can mass and drop in the solutions immediately). • Label all beakers that are using/will use

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