Advanced Gas Measurement of Bio-Pharma Processes IFPAC 2004 Presentation January 15, 2004

1. Advanced Gas Measurement of Bio-Pharma ProcessesIFPAC 2004PresentationJanuary 15, 2004 Ronald R. Rich, President Atmosphere Recovery, Inc. 15800 32nd Avenue North, Suite 110 Plymouth, MN 55447 Ph: (763) 557-8675 Fax: (763) 557-8668 Web: www.atmrcv.com E-mail: rrr@atmrcv.com

2. Company Background • Founded 1994 - Dana Corporation & DOE R&D • Heat Treating Furnace Processes • Grant & Contract Funding • 1995-1998 - Process Gas Recycling System Development • 1997-2000 - Laser Raman Gas Analyzer & Gas Processing Development • 2000-2001 – Analyzer/Controller Field Trials • 2002- – Furnace Analyzer Offerings • 2003- – Bio-Pharma Analyzer Offerings

3. Significant Process Industries - Gas Based • Metal Processing – Initial Success • Automotive & Aerospace Heat Treating • Metal Refining & Powdered Metal • Many Others – Ready for Trials • Bio-Pharma • Petrochemical • Semiconductor • Energy Utilities • Glass & Ceramic • Continuous Emission Monitoring

4. Bio-Pharma Process Goals – General • Lower Production Costs • Higher Productivity and Product Yields • Reduced Feedstock Use • Improved Consistency & Quality • Capital Avoidance (Lower Vessel Numbers) • Other Factors • New Processes & Materials • Lower Analyzer Cost of Operation • Reduced Process Air Emissions & Energy • 12 Month Payback (Max.)

5. Process Gas Conceptual Needs –Better Control, Less Use Off Gases & Vapors Analysis & Control Feedstock Liquids & Solids Off-Line Analysis High Use (H) Std. Infrared Adds Control Med. Use (M) Biological Process Reactor Process Gases (O2) and Liquids (Vapors) Complete Gas Control/Reuse Low Use (L)

6. Metal Processing Gases – Similar Constituents • Carburizing, Carbonitriding, FNC & Nitriding • N2, CO, H2, CO2, H2O, CH4, O2, NH3, CH3OH • Atmosphere Tempering and Annealing • N2, H2, CO, CO2, H2O, CH4, O2, NH3, Ar • Steel, Copper and Aluminum Brazing • N2, H2, CO, CO2, H2O, CH4, O2, NH3, Ar • Powdered Metal Sintering and Annealing • H2, N2, CO, CO2, H2O, CH4, O2,NH3, H2S

7. Bio-Pharma Off-Gas Applications –Similar Needs • CO2, O2, - Routinely Sampled • N2, H2, NH3 – Sometimes Desired Now • Organic Vapors – Very Important • Alcohols, Ketones, Aldehydes, Etc. • Other Inorganics – Determine Rates • S, N, P and Other Compounds • Water Vapor – Determines Balance

8. Typical Process Gas Control - Measures Only One or Two Species • Example Types • Paramagnetic Oxygen Probe – Measures Oxygen • Infrared – Measures Carbon Dioxide • Electrochemical Cells – Low Range Single Gases • Thin Film Technologies – Too Many Interferences • Benefits • Proven Technology (Typically) • Lower Capital Cost • Low Complexity? (Two Analyzers Needed) • Disadvantages • Other Gas Constituents Assumed (Guessed) • Assumptions Often Wrong • Least Accurate Process Control Option • Limits Process Control Options & Improvements

9. Improved Process Gas Control –Absorption-Based Optical (IR) • Can Measure Multiple Gas Species • Carbon Dioxide • Methane • Alcohols (Some) • Ketones (Maybe) • Aldehydes (?) • Benefits • Proven Technology and Vendors • Can be Used to Reduce Feedstock Use Somewhat • Disadvantages • Cannot Measure Diatomics (O2, H2, N2, Etc.) • Detectors Have Limited Measurement Range • Requires Frequent Calibration • Species Measurement Has Significant Overlap • Restricts Optimal Control

10. Other Gas Analysis Technologies –Higher Cost of Ownership • Gas Chromatography (GC) • High Installed Capital Cost ($25,000 - $60,000+) • Slow (2 Minutes+) • Complex – Use Requires Training • Carrier Gas and Frequent Calibration • Laboratory and Petrochemical Processes Predominate • Mass Spectroscopy (MS) • Higher Capital Cost ($50,000 - $120,000) • Requires Vacuum Pump • Gas Mixtures Often Require Second Analysis Method • Ionizer Susceptible to Water Damage • Expensive Sampling System • Expensive to Maintain

11. Ultimate Process Control Goal –Practical Complete Gas Analyzer • Measure All Reactive Gas Species • Detector Range - Low PPM to 100% • Work with Elevated Sample Temperatures • Fast Response • Compact and Operator Friendly • Rugged, Reliable, Easy to Service • Minimal Calibration • Low Cost of Ownership • Potential for Miniaturization

12. Laser Raman Gas Spectroscopy - Features • Unique Frequency “Shift” for Each Chemical Bond • Little Interference Between Most Gases • Measures Gases of All Types (Except Inerts) • Rapid “Real Time” Response Rates Possible • Signal Directly Proportional to Number of Gas Atoms • PPM-100% Gas Concentrations with One Detector • Resolution and Accuracy Depends On: • Laser Power and Optics Variation • Gas Concentration and Pressure • Molecular Bond Type • Background and Scattered Radiation • Optical and Electronic Detector Circuitry

13. Gas to be Analyzed In Special Particle Filter 8 Optical Filters/Sensors (1 for Each Gas Measured) Gas Sample Tube Detector Assembly Laser Beam Plasma Cell Mirror Prism & Mirror Polarizer Gas Out Gas Out Core of Laser Gas Control – Unique 8 Gas Detector Module

14. Detector Module Features • Internal Cavity-Based Raman • Low Power Laser (Helium-Neon Plasma) • Sample Gas Flows Through Instrument • Higher Inherent Accuracy • Discrete Optical Filtering and Quantifying • Any 8 Gases Detected Per Module • Process Specific Configurations & Module #s • Simultaneous Detection of All Gas Species • Fast Detector Updates (50 milliseconds) • Only High Nitrogen Dioxide Levels Interfere • Array Based Interference Computations • 10 Minute Module Exchange

15. Gas Species Lower Limit Hydrogen - H2 10-50 ppm* Nitrogen - N2 50 ppm Oxygen - O2 50 ppm Water Vapor - H2O 10-50 ppm* Carbon Monoxide - CO 50 ppm Carbon Dioxide - CO2 25 ppm Organics - CxHy 10-50 ppm* Ammonia - NH3 10-50 ppm* Typical Gas Constituents Monitored and Detection Limits *Customer Selectable – Selecting Lower Value Limits The Upper Range to 30%; Other Gas Species Substitutable as Options

16. Gas Analyzer – Current Subsystem Integrated Computer & Control System Detector Assembly Sample Pump, Valves and Pressure Control

17. Subsystem Features • Integrated Sampling and Calibration System • Internal Pump and Valves • Low Volume Sample Gas Flows (200 ml/minute) • Multiple Sample Port Options • Automated Zero and Span Calibration • Automated Sample Line Monitoring (Flow & Pressure) • Integrated Electronics & Software • Pentium III Computer w/ HMI and Data Trending • Customizable Process Deviation Analysis • Local and Remote Displays and Interfaces • OPC Server and Client for Connectivity • Available Analog and Digital I/O Options • Multiple Configurable Process and PLC Interfaces • NeSSI Integration Now • NeSSI Generation II Potential

18. Example Main Control Screen

19. Remote Analyzer/Controller – 4 Port Bio-Pharma Product Model 4FM Fermentation Off-Gas Analyzer Outside View Inside View

20. Mobile Process Audit Analyzer – 4 Samples, 8 Pressures, 8 Temperatures • Bio-Pharma Commissioning • Performance Problem Resolution • Advanced Control Demonstration and Testing • ARI Consulting Service

21. Analyzer/Controller – Eight Gases, Four Process Tanks Outside View Inside View

22. Analyzer/Controller – 16 Gases, 16 Process Tanks Outside View Inside View

23. Products Integrate Sampling System & Fully Automated Operation • Fully Integrated Sample System (1-16 Ports) • “Real Time” On-Line Monitoring and Control (1 to 15 Second to Update Each Sample Location) • Operates with Existing PLCs and Sensors • Low Volume Sample Gas Flows (200 ml/minute) • Electronic Flow and Pressure Monitoring • Optics Protection and Enclosure Inerting • Sample Line Pre-Purge and Back-flush Options • Automatic Condensate Removal • Precision Temp. Controlled NEMA Enclosures • Self-Monitoring of Critical Functions • Many Wired and Wireless Communication Options

24. Economic Benefits of High-SpeedGas Process Analysis and Control • Multiple Gas Analysis Capability = System Versatility • Economic Paybacks in Many Ways • Increase Production Capacity • Improve Product Quality • Improve Product Consistency • Reduce Analysis Costs • Reduce Instrumentation and Control Complexity • Reduce Lost Batch Costs • Better Process Documentation • Maintenance Early Warnings • Enhanced Process Safety • Reduce Energy Costs Depends on System Functions Used

25. Thank You For Listening • Looking for Demonstration Sites • Looking for Technology, Marketing & Financial Partners • Brochures if Interested • Questions?