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Andre Salazar Olga A. Zabotina Biochemistry Department Iowa State University

Biochemical Characterization of Amaranthus Cell Wall as a Lignocellulosic Biomass Resource in biorenewable Energy Production. Andre Salazar Olga A. Zabotina Biochemistry Department Iowa State University. Amaranthus – Biomass Resource. Amaranthus – Food Production Agricultural seed

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Andre Salazar Olga A. Zabotina Biochemistry Department Iowa State University

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  1. Biochemical Characterization of Amaranthus Cell Wall as a Lignocellulosic Biomass Resource in biorenewable Energy Production Andre Salazar Olga A. Zabotina Biochemistry Department Iowa State University

  2. Amaranthus – Biomass Resource • Amaranthus – Food Production • Agricultural seed • Environmentally tolerant and adaptable • Amaranthus – Energy Production • Plant cellulose and monosaccharides • Lignocellulosic ethanol • Amaranthus – Potential Economic Impact • Sustainable Supplement to grain production

  3. Research Objective • Hypothesis • Amaranthus is a viable biomass resource for lignocellulosic ethanol production in comparison with plant resources under evaluation. • Objectives • Determine biochemical carbohydrate properties of Amaranthus cell wall • Compare Amaranthus to other plants proposed as biomass resources in lignocellulosic ethanol production • Improve Amaranthus biomass model with the inclusion of cell wall biochemical properties

  4. Experimental Information • Plants • Amaranthus cruentus – DB98246 • Amaranthus hypochondriacus – PI558499 • Plots • Single row (each plant) – 20 ft long, 5 ft apart • Biochemical Analysis • Plant – Stem diameter and height, and mass • Cell Wall – Cellulose, lignin, monosaccharides • Data Statistical Analysis • Mean Comparison – Anova • Covariance Comparison – Correlation

  5. Summary • Amaranthus Plants • Stem and Leaf measurements – No significant difference • Plant dry and cell wall mass – No significant difference • Cellulose and lignin – No significant difference • Xylose – A. hypochondricus > A. cruentus • Biomass Plant Comparison • Cellulose – No significant difference • Lignin – M. sinesis > switchgrass > Amaranthus • Glucose – Maize > M. sinesis, switchgrass , Amaranthus • Xylose – Switchgrass > M. sinesis, Maize > Amaranthus • Galactose – Amaranthus > M. sinesis, Maize, switchgrass • Correlation – Plant measurement to mass

  6. Conclusions • Amaranthus Plants • No significant difference • Xylose content • Biomass resource • Amaranthus comparable to other resources • Cellulose – Equivalent content • Lignin – Less content • Monosaccharide – Equivalent to Maize stover

  7. Additional Research • Amaranthus • Analyze cell wall biochemical properties at 16 wks • Evaluate results for correlations • Evaluate data fit into biomass model

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