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How to Separate These Objects

How to Separate These Objects. 1. 3. 2. 6. 8. 5. 4. 9. 7. 10. 11. 12. 1. 3. 2. cotton. 8. 4. wood. 5. 6. 8. 4. 7. 5. stone. 9. 10. 11. 12. Shape Size Density. wood stone cotton wood wood cotton stone wood stone cotton stone cotton. Shape. 4. 6. Size.

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How to Separate These Objects

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  1. How to Separate These Objects 1 3 2 6 8 5 4 9 7 10 11 12 1 3 2 cotton 8 4 wood 5 6 8 4 7 5 stone 9 10 11 12 Shape Size Density wood stone cotton wood wood cotton stone wood stone cotton stone cotton Shape 4 6 Size 8 5 Density 7 Different rolling speed Different sedimentation Sieving different sizes Juang RH (2004) BCbasics

  2. Basic Principles of Protein Purification Cell Macromolecule Organelle Homogenization Small molecule Cell Debris Amino acid, Sugar, Nucleotides, etc Protein Nucleic Acid Carbohydrate Lipid Ammonium sulfate fractionation The following techniques listed in each box can be used to separate proteins based upon the following characteristics: Size Charge Polarity Affinity Ion exchange, Chromatofocusing, Disc-PAGE, Isoelectric focusing Reverse phase chromatography, HIC, Salting-out Gel filtration, SDS-PAGE, Ultrafiltration Affinity chromatography, Hydroxyapatite Juang RH (2004) BCbasics

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