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pGLO followup and term 4!!!

pGLO followup and term 4!!!. Announcements:. Before April Break you must schedule a 5 minute conference with me after school or at 7:45. List is on the board. This is a HW assignment to set goals for term 4! pGLO lab is due Friday 4/11

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pGLO followup and term 4!!!

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  1. pGLOfollowup and term 4!!!

  2. Announcements: • Before April Break you must schedule a 5 minute conference with me after school or at 7:45. List is on the board. This is a HW assignment to set goals for term 4! • pGLO lab is due Friday 4/11 • Extra help is available next Tuesday from my senior helpers! You must have a draft to work with!

  3. Questions to consider for introduction: • What do the terms “bioengineering” and “cloning” mean? • Where is the gene originally from? • What does the plasmid contain? • How will we get the plasmid into the bacteria? • What will activate the production of GFP in bacteria?

  4. Peer Review of Introduction • Advanced: Discusses concepts and links to the specific goal in this lab!!! • Proficient: Discusses bioengineering, cloning, plasmids and heat shock. Links them to eachother but does not tie to the purpose of bioengineering in this lab. • Needs Improvement: Describes most of the concepts but does not link them to eachother…or…misses concepts • Give your partner an assessment and write a sentence or two to explain why!

  5. Collection of Data • For the purposes of this lab, we are going to call transformation efficiency the number of colonies produced! • You need a table to show the results on all four of your plates as well as a graphical representation (picture, etc). • For class data, we will look at our “experiment within an experiment” • Temperature of “cloning procedure” • Transformation efficiency • How could you choose to display these results?

  6. Conclusions: • What did we learn about the plasmid system we used based on our results? • What were some reasons for variation within the data…is there anything we could modify? • What else could apply our new found knowledge of bioengineering to? • Use the guiding questions on your lab sheet but don’t let them limit you!!!

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