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T hermus AQ uaticus DNA polymerase

T hermus AQ uaticus DNA polymerase.

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T hermus AQ uaticus DNA polymerase

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  1. ThermusAQuaticus DNA polymerase • SEQUENCE:MRGMLPLFEPKGRVLLVDGHHLAYRTFHALKGLTTSRGEPVQAVYGFAKSLLKALKEDGDAVIVVFDAKAPSFRHEAYGGYKAGRAPTPEDFPRQLALIKELVDLLGLARLEVPGYEADDVLASLAKKAEKEGYEVRILTADKDLYQLLSDRIHVLHPEGYLITPAWLWEKYGLRPDQWADYRALTGDESDNLPGVKGIGEKTARKLLEEWGSLEALLKNLDRLKPAIREKILAHMDDLKLSWDLAKVRTDLPLEVDFAKRREPDRERLRAFLERLEFGSLLHEFGLLESPKALEEAPWPPPEGAFVGFVLSRKEPMWADLLALAAARGGRVHRAPEPYKALRDLKEARGLLAKDLSVLALREGLGLPPGDDPMLLAYLLDPSNTTPEGVARRYGGEWTEEAGERAALSERLFANLWGRLEGEERLLWLYREVERPLSAVLAHMEATGVRLDVAYLRALSLEVAEEIARLEAEVFRLAGHPFNLNSRDQLERVLFDELGLPAIGKTEKTGKRSTSAAVLEALREAHPIVEKILQYRELTKLKSTYIDPLPDLIHPRTGRLHTRFNQTATATGRLCCCDPNLQNIPVRTPLGQRIRRGFIAEEGWLLVALDYSQIELRVLAHLSGDENLIRVFQEGRDIHTETASWMFGVPREAVDPLMRRAAKTINFGVLYGMSAHRLSQELAIPYEEAQAFIERYFQSFPKVRAWIEKTLEEGRRRGYVETLFGRRRYVPDLEARVKSVREAAERMAFNMPVQGTAADLMKLAMVKLFPRLEEMGARMLLQVHDELVLEAPKERAEAVARLAKEVMEGVYPLAVPLEVEVGIGEDWLSAKE by Dave Phillis Spring 2010

  2. Dna Replication

  3. PCR Reaction

  4. Helices and Beta Barrels and side chains

  5. DNA and “O” helix

  6. Magnesium metal

  7. Magnesium Interactions

  8. ALL ZINC

  9. Hydrogen Bonding CTP

  10. HYDROPHOBIC INTERACTIONS

  11. ALL HYDRO AND H-BOND

  12. ALL CTP

  13. Sequence Alignment

  14. Sequence Alignment

  15. Literature Cited 1. , F.; Stoffel, S.; Saiki, R.; Myambo, K.; Drummond, R.; Gelfand, D., (1989) Isolation, Characterization, and Expression in Escherichia coli of the DNA Polymerase Gene from Thermusaquaticus.  Journal of Biological Chemistry. 264:6427-6437.2. Patel, P.; Kawate, H.; Adman, E.; Loeb, L., (2000) A single highly mutable catalytic site amino acid is critical for DNA polymerase fidelity.  Journal of Biological Chemistry. Nov 7. 3. Steitz, T.  (1999) DNA Polymerases: Structural Diversity and common Mechanisms.  Journal of Biological Chemistry. 274: 17395-17398. 4. Urs, U.; Ramachandran, M.; Krishna Murthy, H.,  (1999)  Structure of Taq polymerase shows a new orientation for the structure-specific nuclease domain.  ActaCryst. D55:1971-1977. 5. Villbrant, B. ; Sobek, H. ; Frey, B.; Schomburg, D.,  (2000)  Domain exchange: chimeras of thermusaquaticus DNA polymerase, escherichia DNA polymerase I and thermotoganeapolitana DNA polymerase.  Protein Engineering.  9:645-54. 6. Kim, Y.; Eom, S.; Wang, J.; Lee, D.; Suh, S.; Steitz, T.,  (1995)  Crystal structure of Thermusaquaticus DNA polymerase Nature 376, 612-616 (17 August 1995)7. Chien A, Edgar DB, Trela JM (1976). "Deoxyribonucleic acid polymerase from the extreme thermophileThermusaquaticus". J. Bact. 127 (3): 1550–7. 8. Saiki, RK; et al. (1988). "Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.". Science 239 (4839): 487–91.9. Saiki, RK; et al. (1985). "Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia"Science 230 (4732): 1350–410. Lawyer FC, et al. (1993). "High-level expression, purification, and enzymatic characterization of full-length Thermusaquaticus DNA polymerase ...". PCR Methods Appl. 2 (4): 275–87.11. Tindall KR and Kunkel TA (1988). "Fidelity of DNA synthesis by the Thermusaquaticus DNA polymerase". Biochemistry 27 (16): 6008–13

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