From turned off…

1. From turned off… ..to tuned in Tips to construct and display complex research data Most Postdoctoral researchers see the difference. Those who follow these tips make a difference. (Start the Slide Show. This presentation is automatically timed: 3.75 minutes)

2. Components of the canonical JNK pathway in dorsal closure are required for wound closure canonical JNK pathway ? Txn factors MAP4K MAP3K MAP2K MAPK control MAP4K msn 100 slpr MAP3K 80 hep MAP2K 60 open wounds [%] MAPK bsk 40 20 15 Txn factors DJun DFos † † 0 w1118 slpr Takl2 Mkk4 Takl1 bsk x2 Tak1 x2 hep x2 Pk92B Mekk1 msn x2 DJun DFos response wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥ 30 † lethal cross Here’s the original slide ^ Oversized headline sucks almost a third of the space? Navigation/legend sucks >half of what remains? < Leaving only 25% of space for the research data!?

3. Components of the canonical JNK pathway in dorsal closure are required for wound closure canonical JNK pathway 1 +6 ? +7 Txn factors MAP4K MAP3K MAP2K MAPK control MAP4K msn 100 slpr MAP3K 80 +5 +4 +23 hep MAP2K 60 open wounds [%] MAPK bsk +9 +10 40 +11 20 15 Txn factors DJun DFos † † 0 +17 w1118 slpr Takl2 Mkk4 Takl1 bsk x2 Tak1 x2 hep x2 Pk92B Mekk1 msn x2 DJun DFos response +13 wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥ 30 † lethal cross Over 106 elements scream for attention

4. 100 80 canonical JNK pathway 60 open wounds [%] 40 Txn factors MAP4K MAP3K MAP2K 20 MAPK control 15 MAP4K 0 MAP3K MAP2K MAPK w1118 slpr Takl2 Mkk4 Takl1 bsk x2 Tak1 x2 hep x2 Pk92B Mekk1 msn x2 DJun DFos Txn factors † † N = ≥ 30 † lethal cross wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 Components of the canonical JNK pathway in dorsal closure are required for wound closure ? And here’s how it was animated msn slpr hep bsk DJun DFos response

5. This is what’s called aTreasure Hunt Audiences take in data fromtop-to-bottom, left-to-right Let’s rebuild this slide,starting with a roadmap

6. Canonical JNK pathway Canonical JNK pathway ? ? MAP4K MAP4K msn msn MAP3K MAP3K slpr slpr MAP2K MAP2K hep hep MAPK MAPK bsk bsk Txn factors Txn factors DJun DFos DJun DFos response response Components of canonical JNK pathwayin dorsal closure are required for wound closure First display the title:“what we’ll discuss” Our roadmap Next: “how we plan to get there”(organization of data sets) And we plan to share itin reverse order Now! We’re ready to share data

7. % openwounds Txn factors MAPK MAP2K MAP3K MAP4K 100 80 60 40 20 15 0 Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † genes Components of canonical JNK pathwayin dorsal closure are required for wound closure If you don’t explain chart features BEFORE displaying the data, expect to lose half your audience

8. Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † control w1118 Components of canonical JNK pathwayin dorsal closure are required for wound closure % openwounds Txn factors MAPK MAP2K MAP3K MAP4K 100 80 60 40 20 15 0 Make a point

9. Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † control † w1118 DJun DFos Components of canonical JNK pathwayin dorsal closure are required for wound closure % openwounds Txn factors MAPK MAP2K MAP3K MAP4K 100 80 60 40 20 15 0 Make a point

10. Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † control † w1118 DJun DFos bskX2 Components of canonical JNK pathwayin dorsal closure are required for wound closure % openwounds Txn factors MAPK MAP2K MAP3K MAP4K 100 80 60 40 20 15 0 Make a point

11. Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † control † w1118 DJun DFos bskX2 hepX2 Mkk4 Components of canonical JNK pathwayin dorsal closure are required for wound closure % openwounds Txn factors MAPK MAP2K MAP3K MAP4K 100 80 60 40 20 15 0 Make a point

12. Wound reporter: e22c-Gal4 A58-Gal4 Dcr-2;A58-Gal4 N = ≥30 N = lethal cross † control † Takl1 Takl2 Pk92B w1118 DJun DFos bskX2 hepX2 slpr Tak1X2 Mkk4 Mekk1 Components of canonical JNK pathwayin dorsal closure are required for wound closure % openwounds Txn factors MAPK MAP2K MAP3K MAP4K 100 80 60 40 20 15 0 Make a point

13. Wound reporter: E22c-Gal4 A58-Gal4 Dor-2; A58-Gal4 N = ≥30 N = lethal cross † control † † Takl1 Takl2 Pk92B msnX2 w1118 DJun DFos bskX2 hepX2 slpr Tak1X2 Mkk4 Mekk1 Components of canonical JNK pathwayin dorsal closure are required for wound closure % openwounds Txn factors MAPK MAP2K MAP3K MAP4K 100 80 60 40 20 15 0 Make a point

14. Components of canonical JNK pathwayin dorsal closure are required for wound closure % openwounds Txn factors MAPK MAP2K MAP3K MAP4K 100 80 60 40 20 15 0 Wound reporter: E22c-Gal4 A58-Gal4 Dor-2; A58-Gal4 N = ≥30 N = lethal cross † control † † Takl1 Takl2 Pk92B msnX2 w1118 DJun DFos bskX2 hepX2 slpr Tak1X2 Mkk4 Mekk1 Axis labels replace legends Graphic boxes move the eye to the next point > left-to-right Animations and transitions focus on the point being made Purposeful builds keep the audience tuned in Follow these guidelines;keep audiences turned on More tips > www.stevetoms.net/powerpoint.htmSteve Toms, U.T. Communications Coach713-662-6040 | steve@stevetoms.netHit Space Bar or “Escape” to end