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iGEM Meeting

iGEM Meeting. 3-19-2010. Brochure and Sponsorship Package. Lab Update Regional Conference Contacts for further publicity. Dave Leake from Surrounded by Science . Credit Hours Safety information? No meeting Next week – Happy Spring Break! Movie Night April 1 st. Francis’ Lab Work.

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iGEM Meeting

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  1. iGEM Meeting 3-19-2010

  2. Brochure and Sponsorship Package. • Lab Update • Regional Conference • Contacts for further publicity. Dave Leake from Surrounded by Science. • Credit Hours • Safety information? • No meeting Next week – Happy Spring Break! • Movie Night April 1st

  3. Francis’ Lab Work • sRNA resubmission • MicC Mutagenesis

  4. sRNA Resubmission • Standardization • All target sequences contain an NheI site • All target sequences contain one of the following (EcoRI, SpeI, PstI, XbaI) • Site directed mutagenesis • Each isocaudamer site must be removed before submission

  5. micC Mutagenesis E. Coli micC - ompC S. Typhimirium micC – ompC, ompD

  6. EGFP Knockout • 6 MicC mutants • 5’ END • 3’ Terminator • RBS • +67 site • Random

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