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ASLA Biotech Ltd. SME partner for EU

ASLA Biotech Ltd. SME partner for EU. www.asla-biotech.com Established in 1999 8 permanent positions 10 collaborating research fellows Anatolijs Sharipo, CEO. FP 7 meeting 13 SEP 200 7. ASLA Biotech Ltd. currently participates in two EU FP6 projects as a partner:

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ASLA Biotech Ltd. SME partner for EU

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  1. ASLA Biotech Ltd. SME partner for EU www.asla-biotech.com Established in 1999 8 permanent positions 10 collaborating research fellows Anatolijs Sharipo, CEO FP7 meeting 13 SEP 2007

  2. ASLA Biotech Ltd. • currently participates in • two EU FP6 projects • as a partner: • COMPUVAC: Rational design and standartized evaluation of novel genetiv vaccines Integrated Project • BacAbs: Assesment of structural requirements in complement-mediated bactericidal events FP7 meeting 13 SEP 2007

  3. our experience: STAGES OF SUCCESS To find partner To participate in proposal To get finansed To do the job FP7 meeting 13 SEP 2007

  4. To find partner • How to find a partner: • Personal links: experience in international research • CORDIS databases • - registration and waiting for searchers • - active search • National Point for FP grants FP7 meeting 13 SEP 2007

  5. To find partner To participate in proposal • How to convice coordinator and other partners in your participation in the consortium?: • Demonstrate expertize (you have to have it first) • Find a nishe (there has to be one in the project) • Show advantages by: • your quality • your budget • Take initiative if necessary • Do not take initiative if not necessary FP7 meeting 13 SEP 2007

  6. To find partner To participate in proposal Quality as a Partner • Liepinsh E, Barbals R, Dahl E, Sharipo A, Staub E, Otting G. The death-domain fold of the ASC PYRIN domain, presenting a basis for PYRIN/PYRIN recognition. J Mol Biol. 2003 Oct 3;332(5):1155-63. PMID: 14499617 [PubMed - indexed for MEDLINE] • Liepinsh E, Leonchiks A, Sharipo A, Guignard L, Otting G. Solution structure of the R3H domain from human Smubp-2. J Mol Biol. 2003 Feb 7;326(1):217-23. PMID: 12547203 [PubMed - indexed for MEDLINE] • Williams NK, Prosselkov P, Liepinsh E, Line I, Sharipo A, Littler DR, Curmi PM, Otting G, Dixon NE. In vivo protein cyclization promoted by a circularly permuted Synechocystis sp. PCC6803 DnaB mini-intein. J Biol Chem. 2002 Mar 8;277(10):7790-8. Epub 2001 Dec 12. PMID: 11742000 [PubMed - indexed for MEDLINE] • Liepinsh E, Baryshev M, Sharipo A, Ingelman-Sundberg M, Otting G, Mkrtchian S. Thioredoxin fold as homodimerization module in the putative chaperone ERp29: NMR structures of the domains and experimental model of the 51 kDa dimer. Structure (Camb). 2001 Jun;9(6):457-71. PMID: 11435111 [PubMed - indexed for MEDLINE] • Publications with our participation as authors • References to our products and services Papers referring ASLA Biotech as a labeled protein/aligning reagents provider: 45 since 2001 Journals:Molecular Cell, Nat Struct Mol Biol.,Structure, J Biomol NMR, Protein Sci., J. Biol. Chem, J. Mol. Biol., Nucleic Acids Research,Biopolymers,RNA, J. Am. Chem. Soc., BMC Cell Biology, www.asla-biotech.com/asla-ref.htm FP7 meeting 13 SEP 2007

  7. To find partner To participate in proposal Facilities & Equipment • Our own facilities • Access to complicated equipment FP7 meeting 13 SEP 2007

  8. ASLA BMC OSI To find partner To participate in proposal Using big academical Institutions Facilities/Infrasructure Scientific environment Research collaboraton FP7 meeting 13 SEP 2007

  9. To find partner To participate in proposal To get finansed • How to win?: • choose right consortium/coordinator • trust them • do the best for the consortium during proposal • do not put all eggs in the same basket • If lost: • do not complain • analyse your negative experience • start from the step 1 FP7 meeting 13 SEP 2007

  10. To find partner To participate in proposal To get finansed “To swim, you have to swim” Mao Dze Dung “Impossible is nothing” Adidas “Just do it” Nike • Our recovery of financed grants versus submitted applications: • 1 grant of 3 in 2005 • 2 grants of 13 in 2006 • ? grants of 8 in 2007 FP7 meeting 13 SEP 2007

  11. To find partner To participate in proposal To get finansed To do the job EXPERTIZE we bring to EU consortia Products • NMR aligning reagents • NMR protein standards ready to use, sealed in NMR tubes • Antibodies Custom Services • labeling proteins by 13C, 15N and 2H • artificial gene synthesis • molecular biology services • custom immune reagents Complex customer oriented studies • artificial gene synthesis -> specific reagent production • subcellular localization for the molecule of interest FP7 meeting 13 SEP 2007

  12. To find partner To participate in proposal To get finansed To do the job Protein Expression and Purfication • proteins from native sources • bacterially expressed proteins: • fusion proteins: GFP, GST, RSA, His-tag, MBP, STREP • non-tagged proteins • proteins expressed in insect/mammalian cell cultures FP7 meeting 13 SEP 2007

  13. To find partner To participate in proposal To get finansed To do the job Protein Expression and Purfication Optimization of expression: temperature, medium, E.coli strain etc. E.Coli growth scales: Flask expression 4L 4ML fermentor 20L fermentor from subcontractors Labeling: 15N, 13C, 2H in all combinations, selective labeling available • Purification: • FPLC/HPLC: ion exchange, size exclusion, reverse phase • Affine chromatography • Analysis: • PAAGE – coomassie/silver stain • Mass spectrometry • WB/IP/ICC validation

  14. To find partner To participate in proposal To get finansed To do the job Protein Expression and Purfication: labeled ubiquitin NMR standards 15N-1H HSQS 15N-labeled ubiquitin

  15. To find partner To participate in proposal To get finansed To do the job Custom Immune ReagentsPolyclonal Sera & mAbs • Antigens: • proteins from native sources • bacterially expressed proteins • proteins expressed in mammalian cell cultures • peptides coupled to different carriers • fusion proteins: GFP, GST, RSA, His-tag, MBP, STREP FP7 meeting 13 SEP 2007

  16. To find partner To participate in proposal To get finansed To do the job NMR studies • Concept:We provide contract studies on protein structure determinations and other experiments related to the protein structure and interactions. We express, label by stable heavy isotopes and purify the protein of your interest and finally, we are able to determine its structure by NMR spectroscopy methods. The service is provided on commercial basis. • Equipment: a permanent access to 4 chanel ( 1H, 13C, 15N, 2D) NMR spectrometer Varian INOVA 600 MHz equipped with cryoprobe located at the Latvian Institute of Organic Synthesis • NMR expertize: Dr Edvards Liepinsh, OSI, NMR of proteins and nucleic acids FP7 meeting 13 SEP 2007

  17. To find partner To participate in proposal To get finansed To do the job BE FLEXIBLE • VERY OFTEN THE TASK MIGHT BE CHANGED DURING THE PROJECT • ONE NEEDS TO BE READY FOR NEW TASKS IN FRAMES OF THE EXPERTIZE • TO BE READY TO GET NEW EXPERTIZE FP7 meeting 13 SEP 2007

  18. Ainars Leonchiks, PhD Marina Koulikovska, PhD Anatoly Sharipo, PhD Gunda Grinberga Marija Teveleva Sabine Skipare Zhanna Rudevica Gunda Grinberga Victoria Logina Dima Zhulenkovs Andris Zeltinsh, PhD Janis Bogans

  19. ASLA BIOTECH LTD www.asla-biotech.com Latvian Biomedicine Research and Study Centre, Ratsupites 1 LV-1067, Riga, LATVIA

  20. Thank you FP7 meeting 13 SEP 2007

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