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Studies on the role of aromatic shrubs in the inhibition of adjacent vegetation. Kotoulas V., Panagopoulos G., Skouras V., Economou G. and Karamanos J.A. Laboratory of Agronomy, Faculty of Crop Science, Agricultural University of Athens. The biodiversity of aromatic flora in Greece
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Studies on the role of aromatic shrubs in the inhibition of adjacent vegetation. Kotoulas V., Panagopoulos G., Skouras V., Economou G. and Karamanos J.A. Laboratory of Agronomy, Faculty of Crop Science, Agricultural University of Athens.
The biodiversity of aromatic flora in Greece Typical constituent element of Mediterranean ecosystem The state of the art
Origanumhirtum Origanumonites The state of the art
Coridothymuscapitatus Saturejathymbra The state of the art
The classic uses - herbs - medicins - essential oils The state of the art • "Letfoodbethymedicineandmedicinebethyfood" Hippocrates, theFatherofMedicine • (460-377 B.C.)
The novel uses (industrial use) - pharmaceutics - cosmetics, - agriculture - antimicrobial - animal production The state of the art
the state of the art • agriculture - antimicrobial • - animal production QUESTION ? Inhibitory effect on weeds ?
The inhibitory effect has been stated on oregano Observation in situ hYPOTHESIS
To study the inhibitory effect of all the “origanum” species indigenous of Greek ecosystems and Mediterranean in general The longterm aim their effective use in terms of weed control Scope
Objectives In situ assesement of inhibitory effect In vitro biotests of their phytotoxicity Approaching the scope………..
Islands of the north-eastern Aegean sea Ikaria and Fournoi 4 aromatic species Origanumonites Origanumhirtum Coridothymuscapitatus Saturejathymbra Case study
Study of adjacent vegetation during surveys in localized aromatic habitats. Plot area: 15 m2 (5m x 3m) Richness: Number of the species Coverage: (the vertical projection of plant canopy on the ground) Importance value: frequency + coverage (Wikum and Shanholtzer, 1978) Methodology
MEthodology Dose respone of herbal distillates • Biotests a. Avenaradicle Determination of 50% inhibition in radicle elongation b. Spirodelapolyrhizachlorosis Determination of 50% Lethal Dose (LD50%) Doses: (ml of herbal distillate/ml of dilution) 1 (A) 0.5 (A/2) 0.25 (A/4) 0.125 (A/8) 0.062 (A/16) 0.031 (A/32) 0.015 (A/64) 0.008 (A/128) Doses: (ml of herbal distillate/ml of dilution) 1 (A) 0.5 (A/2) 0.25 (A/4) 0.125 (A/8) 0.063 (A/16)
Richness Results
ResultsImportance value a. Origanum onites
ResultsImportance value b. Origanum hirtum
ResultsImportance value c. Coridothymus capitatus
ResultsImportance value d. Satureja thymbra
In situ observations Lower richness in O. hirtum Lower importance value in O. onites Overview conclusions
Biotests O. hirtun showed the higher inhibitory effect Overview conclusions
The identification of the effective constituent The future effective use The effective application on farm for weed control Key messages