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Sampling For Success

Sample collection & submission, chain of custody, choosing the right test for Microbiological testing. Sampling For Success. Common Types of Microbiological Samples for Food Plants. Swabs (stick, sponge, cloth) Bulk – Raw materials, Finished Products Water.

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Sampling For Success

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  1. Sample collection & submission, chain of custody, choosing the right test for Microbiological testing Sampling For Success

  2. Common Types of Microbiological Samples for Food Plants • Swabs (stick, sponge, cloth) • Bulk – Raw materials, Finished Products • Water

  3. Preparing to Collect Swab Samples • Appropriate type and amount of swabs • Labelling materials • Sanitizer • Neutralizing buffer • Chain of custody • Sampling plan

  4. Types of Swabs • Stick • Sponge • Cloth

  5. Swabbing Technique • Label swab, noting sampling location • Open swab aseptically and swab sample site • Close swab and sanitize swab site • Refrigerate swabs until transport to laboratory

  6. Preparing to Collect Product Samples • Sterile Sampling Materials • Spoons, forceps, knives • Sample bags or containers • Gloves, other protective apparel as required • Labelling Materials • Chain of Custody • Sampling Plan

  7. Product Sampling Guidelines • Collect unopened containers if possible • If collecting from bulk containers, ensure sample is representative of the product being sampled • Make sure to collect at least enough sample to meet analysis requirements • Store samples at appropriate temperature in preparation for delivery to the laboratory

  8. Planning for Collecting a Water Sample: • Sterile Sample Bottles • preservative • Labels • Chain of Custody • Cooler and Ice Packs • Transportation Arrangements

  9. Water Sample Site Considerations Sites to Avoid: • Dead end mains • Seldom used faucets • Faucets with a point of use filter eg. Carbon • Garden or other flexible hoses

  10. Collecting the Water Sample • Pre-label the sample container -Date, time of sample, site location • Remove any aerators or screens from the faucet • Run the faucet cold for at least 2 minutes • Open the sample bottle and collect the water until the shoulder, leaving a headspace • Seal the sampling container tightly

  11. Preparing Samples for Shipping to the Laboratory • Place samples in a cooler with an ice pack • Do not allow water samples to freeze • Avoid loose ice • Include Complete Chain of Custody (COC) • Label cooler clearly with site location and destination laboratory location

  12. Important Components of a Chain of Custody (COC) • Identifies sender and receiver of sampled materials • Specifies analyses and methodologies to be performed on samples • Provides descriptions of samples for reporting of analysis results • Serves as a legal document for the traceability of the handling and possession of samples

  13. Points to Remember when Completing COCs • Contact information for company and sampler is available • Sample descriptions allow for the easy identification of the samples included • Analysis and methodology indicated • Sampling plan requirements clearly stated

  14. Chain of Custody (Water) • Client Information • Sample Site Identifications • Name of Sampler • Date and time of sample collections • Analysis Requested • Drinking/ Non-drinking water

  15. Choosing the Right Test • Consider the Objectives of Testing: • Sanitation monitoring • Standard Plate Count, Coliforms, Yeast & Mold • Regulatory compliance • Correct method for compliance • Lab accreditation for methodology • Pathogen screening • Appropriate for the product/environment

  16. Importance of Proper Sampling • From J. Moldenauer and Sutton, “Towards an Improved Sterility Test,” Commentary, PDA Journal of Pharmaceutical Science and Technology Odds of catching sterility breakdown in a batch when taking 20 samples for USP sterility test

  17. Thank you Contact: Alex Montgomery IG MicroMed Environmental Inc. 604-279-0666 alex@igmicromed.com

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