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Daniel L. Burden, Chemistry Department, Wheaton College Wheaton, IL 60187

Dissecting the Dynamics of Surfactant Assemblies with Numerical Fluorescence Correlation Spectroscopy. Daniel L. Burden, Chemistry Department, Wheaton College Wheaton, IL 60187.

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Daniel L. Burden, Chemistry Department, Wheaton College Wheaton, IL 60187

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  1. Dissecting the Dynamics of Surfactant Assemblies with Numerical Fluorescence Correlation Spectroscopy Daniel L. Burden, Chemistry Department, Wheaton College Wheaton, IL 60187 Numerical Fluorescence Correlation Spectroscopy (NFCS) follows the general protocol established for traditional Fluorescence Correlation Spectroscopy (FCS), but replaces analytical modeling with two numerical features: (1) a numerical map of the detection volume; (2) a computer simulation that produces correlation curves for scenarios that are difficult or impossible to model analytically. Diffusion within and around nanoconfined spaces is difficult to interpret with traditional FCS due to the small geometric constraints imposed on the fluorescent tracers. The approach under development in our lab allows investigation of nearly any geometric scenario by building obstacles or other diffusion constraints into the simulation code. Thus, the simulator is able to distinguish diffusive components in FCS data that would normally be difficult to interpret.Results from recent work have been published in Langmuir, 25(14), 8330, 2009.

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