1 / 35

Safety in the lab

Safety in the lab. Reminder of (forgotten) procedures General rules Problems. Work safely = Work cleanly = Work accurately. ‘Small accidents’. 2-Cyclohexene-1-one Balance Gloves + rinsed. 24h => Anti-gif, doctors Flammazine Infection => antibiotics + corticoids

howardjohn
Download Presentation

Safety in the lab

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Safety in the lab • Reminder of (forgotten) procedures • General rules • Problems Work safely = Work cleanly = Work accurately

  2. ‘Small accidents’ • 2-Cyclohexene-1-one • Balance • Gloves + rinsed • 24h => Anti-gif, doctors • Flammazine • Infection => antibiotics + corticoids • Hyperpigmentation + allergy?

  3. Experimental design • Start with risk analysis form • http://www.biw.kuleuven.be/ifc/cok/intern/forms.htm • Obligatory!Checked by supervisor! -> printed copy to Ivo Vankelecom • Find information atwww.kuleuven.ac.be/sapredir/gevaarlijkestoffen • Catalogen, boeken • Limit quantities • Use safest possible chemicals (ethanol, octane, toluene!)

  4. Safety spectacles + lab coat • ALWAYS!

  5. Lab tables • Space/clean

  6. Lab tables • Space/clean • Label name + datehttp://www.biw.kuleuven.be/ifc/cok/intern/forms.htm

  7. Lab tables • Space/clean • Label name + datehttp://www.biw.kuleuven.be/ifc/cok/intern/forms.htm • Place back bottles

  8. Lab tables • Space/clean • Label name + datehttp://www.biw.kuleuven.be/ifc/cok/intern/forms.htm • Place back bottles • Whenever volatile products: FUME CUPBOARDS!

  9. Lab tables • Space/clean • Label name + datehttp://www.biw.kuleuven.be/ifc/cok/intern/forms.htm • Place back bottles • Whenever volatile products: FUME CUPBOARDS! • Solvent-waste bottles: FUME CUPBOARDS!Or closed!

  10. Lab tables • Space/clean • Label name + datehttp://www.biw.kuleuven.be/ifc/cok/intern/forms.htm • Place back bottles • Whenever volatile products: FUME CUPBOARDS! • Solvent-waste bottles: FUME CUPBOARDS! • Close fume cupboards

  11. Balances • Precision instrument! • Horizontal • Space • Clean

  12. Waste disposal: 1.Needles • Do not place back needle cap! • Remove needle with red lid of yellow plastic box • Syringe in contaminated waste

  13. Waste disposal: 2. GC-vials • Remove from tray after analysis!

  14. Waste disposal: 2. GC-vials • Remove from tray after analysis! • Special box

  15. Waste disposal: 2. GC-vials • Remove from tray after analysis! • Special box

  16. Waste disposal: 2. GC-vials • Remove from tray after analysis! • Special box • Results not interesting?

  17. Waste disposal: 3. Pasteurpipettes • Contaminated waste

  18. Waste disposal: 4. Product bottles • Rinse + dry + remove cap • Lab 02.92 (new) • Write down (also when bottle remains used!) Plastic bottles+ glass bottles Broken glass

  19. Waste disposal: 5. Chemicals • Liquids: in barrels • More information?=> Lieve

  20. Waste disposal: 5. Chemicals • Powders: in special box

  21. Waste disposal: 5. Chemicals • Contaminated waste: in special box

  22. Dust masks • Whenever powders • Special gas masks:outside labs (mind filter colour!) • CO!

  23. Gloves • Whenever needed • Green rubber!

  24. Warnings (reaction tags) • Use special formshttp://www.biw.kuleuven.be/ifc/cok/intern/forms.htm • Remove them afterwards!

  25. Dish washer • Duty list: Remind eachother ! • Use rubber gloves • No ‘spuwpotjes’ • Glassware should be precleaned: • NO scrubbing machine • NO products left

  26. Centrifuge • Leave it clean!

  27. Miscellaneous I • Never work ALONE in the lab! • Gas connections: check leaks! • Tap connections • Fume cupboard  lab book • Respect eachother’s set-ups • Respect reservations (forms!)

  28. Miscellaneous II • HF: written authorisation! • Avoid ovens to evaporate • Report defects (lab responsible, technician, supervisor) • Liquid nitrogen (gloves!) • Storing samples (ask lab responsible, technician, supervisor) • General lab cleanings

  29. General responsibles

  30. Ordering of chemicals • Forms on website (http://www.biw.kuleuven.be/ifc/cok/intern/forms.htm) • Send e-mail to responsibles • Chemicals: • Consult database! (p:/COK workers/Product Database/db3 -> lievegina) • Internal blue numbers: • cellar • empty bottles

  31. COK-website: intern • Forms • General safety in the lab • Guidelines about safety and ecology in the lab • Risk Analysis form • Fire extinguishers • Reaction tags

  32. COK-website: intern • Reservation forms http://www.biw.kuleuven.be/ifc/cok/intern/forms.htm • Reservation calcination oven • Reservation synthesis oven • Reservation dry oven • Reservation U tube oven • Reservation vacuum oven • Instrument specifications

  33. Emergency procedures • Call 22222 (not 100!) • Fire: 1. Call ‘Fire!’ If people around: -> 2 If no one around: activate alarm, then -> 2 2. 1 attempt to exstinguish the fire (time!) 3. Call 22222 4. If successful: wait for rescue team If not successfull: - activate alarm - go to ‘verzamelpunt’

  34. Evacuations • Continuous siren (+/- 5 min) >< test or automatic detection of fume,…= short pulse (3 seconds) -> meant to activate EIP (first intervention team = Hugo + Johan M.) -> they have 3 minutes to decide ‘accept’ (-> continuous siren -> general evacuation) or ‘decline’ (-> silence) • Switch off electric equipment • (PC, printer can remain switched on) • Leave lights on • Bring experiments in a ‘safe’ mode • Close windows and doors • Leave the building (take jacket, wallet, bag,…) • Do not use the elevator! • Go to reunion point (in front of main entrance of the building) and await further instructions

More Related