1 / 1

PKC d -pTyr 311

0. 0.5. 1. 2. Thr (min). eNOS. PKC d -pTyr 311. PKC d. †. eNOS-pSer 1179. eNOS-pThr 497. eNOS. A. B. *. 125. *. 100. 75. eNOS-p (%). †. 50. 25. 0. Thr. -. +. -. +. -. +. DMSO. Rott. LY. eNOS-pSer 1179. D. †. 150. 125. C.

hu-pace
Download Presentation

PKC d -pTyr 311

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. 0 0.5 1 2 Thr (min) eNOS PKCd-pTyr311 PKCd † eNOS-pSer1179 eNOS-pThr497 eNOS A B * 125 * 100 75 eNOS-p (%) † 50 25 0 Thr - + - + - + DMSO Rott LY eNOS-pSer1179 D † 150 125 C pSer1179 100 † 100 pThr497 eNOS-p (%) 75 75 50 eNOS-p (%) 50 25 0 25 - + + + Thr 0 - - co pre - + - + Rott PMA Basal A23 eNOS-pSer1179 eNOS On Line Figure I. A. HUVECs pretreated with 10 mol/L rottlerin (Rott), 10 mol/L LY294002 (LY), or control vehicle (0.1% DMSO) for 30 min were stimulated with 10 U/mL thrombin (Thr) for 1 min. B. BAECs were stimulated with thrombin for the indicated time periods. PKCd Tyr311 phosphorylation was evaluated by immunoblotting. C. BAECs pretreated with or without 10 mol/L rottlerin (Rott) for 30 min were stimulated with 10 mol/L A23187 for 1 min. D. BAECs co-treated (co) or pretreated (pre 5 min) with 1 mol/L PMA were stimulated with 10 U/mL thrombin for 1 min. These data shown are representative of 3 separate experiments giving similar results (mean + SEM. *p < 0.05 compared to basal. †p < 0.05 compared to stimulated control.).

More Related