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Exposing C.reinhardtii to Anaerobic Atmospheric Conditions to Enhance Hydrogen Production

Exposing C.reinhardtii to Anaerobic Atmospheric Conditions to Enhance Hydrogen Production. Chrisitna George Period 0,1 Even June 1, 2009. Need. http://www.physicalgeography.net/fundamentals/images/co2_atmosphere.jpg. Need. http://www.tspusa.com/images/TICimages/alternativefuels1.gif.

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Exposing C.reinhardtii to Anaerobic Atmospheric Conditions to Enhance Hydrogen Production

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  1. Exposing C.reinhardtii to Anaerobic Atmospheric Conditions to Enhance Hydrogen Production Chrisitna George Period 0,1 Even June 1, 2009

  2. Need http://www.physicalgeography.net/fundamentals/images/co2_atmosphere.jpg

  3. Need http://www.tspusa.com/images/TICimages/alternativefuels1.gif

  4. Knowledge Base • Anaerobic Conditions: the absence of oxygen http://www.ilmvac.co.uk/content/products/Anaerobic-container-p112555-01.html

  5. Knowledge Base • Sulfur Deprivation: Growing the algae in Sulfur-replete medium or Sulfur-free medium http://www.enasco.com/prod/images/products/8A/VC128314l.jpg

  6. Knowledge Base • Autotrophic organism that produces hydrogen in sulfur deprived anaerobic conditions C.reinahrdtii http://www.sciencedaily.com/images/2009/03/090324171556-large.jpg

  7. Knowledge Base Light intensities v. the CO2 exchange when measured by the photosynthetic rate http://generalhorticulture.tamu.edu/lectsupl/Light/p54f1.gif

  8. Isoamylase Gene • Important gene for starch accumulation which is important for hydrogen production (Posewitz, 2005). http://www.mrc-lmb.cam.ac.uk/genomes/date/1bf2.gif

  9. Literature Review • Rosenbaum, Miriam (2005) ‘Utilizing the Green Alga C.reinhardtii for Microbial Electricity Generation” • Direct electricity generation from microbial photosynthetic activity • Oxidative depletion of hydrogen, photosynthetically produced by C.reinahrdtii under sulfur deprived conditions, by polymer coated electro catalytic electrodes

  10. Literature Review • Melis, Anastasios (2000) “Sustained Photobiological Hydrogen Gas Production upon Reversible Inactivation of oxygen Evolution in C.reinhardtii” • As sulfur deprivation increases, so does the hydrogen production

  11. Literature Review • Posewitz, Matthew C. (2004) “Hydrogen Photoproduction is Attenuated by Disruption of an Isoamylase Gene in C.reinhardtii” • Time represents the anaerobic induction time

  12. Literature Review • Logan, Bruce E. (2006) “Using Algae and other Biomass for Hydrogen Production in a Modified Microbial Fuel Cell” • A small voltage was applied (.25 V) to the algae, which generated pure hydrogen gas at the cathode

  13. Purpose • Therefore, the purpose of this experiment is to find the optimal duration and frequency of anaerobic exposure needed to optimize C.reinhardtii hydrogen production

  14. Hypotheses • Alternate: A short period of anaerobic conditions will increase the hydrogen production • Null: The duration and frequency of anaerobic conditions will have no effect on the amount of hydrogen produced

  15. Exposing C.reinhardtiito Anaerobic Atmospheric Conditions to Enhance Hydrogen Production Problem: What is the optimal duration and frequency of anaerobic exposure needed to optimize C.reinhardtii hydrogen production? Control Groups: The Algae growth medium, Temperature, and light intensity Independent Variable: Growth of C.reinhardtii and hydrogen produced Dependent Variable: Carbon dioxide levels, pH levels 6 hours of anaerobic atmospheric conditions 24 hours of anaerobic atmospheric conditions 12 hours of anaerobic atmospheric conditions -Carbon dioxide concentration measured using a Pasco GLX Xplorer -pH levels measured using pH paper -growth of C.reinhardtii measured using a Spectrophotometer -The hydrogen gas produced will be measured using an H2Scan hydrogen detector. -A fluorometer will be used to measure the photosynthetic rate. All data will statistically analyzed by SPSS, by an ANOVA followed by a Sheffe Post Hoc Test. Data will then be put in line graphs.

  16. Do Ability • C.reinhardtii: Can be grown in the lab as done in previous years • Anaerobic container can be purchased • A hydrogen sensor will be difficult to find at a low cost

  17. Budget

  18. Work Cited • Agency for Science, Technology and Research (A*STAR), Singapore. "Carbon Dioxide Transformed Into Methanol." ScienceDaily 17 April 2009. 23 April 2009 <http://www.sciencedaily.com­ /releases/2009/04/090416102247.htm>. • **“Algae Could One Day Be Major Hydrogen Fuel Source.” Science Daily. April 2, 2008. • American Chemical Society. "'Ice That Burns' May Yield Clean, Sustainable Bridge To Global Energy Future." ScienceDaily 24 March 2009. 23 April 2009 <http://www.sciencedaily.com­ /releases/2009/03/090323143858.htm>. • Basque Research. "Obtaining Bio-gas From Food Industry Waste." ScienceDaily 31 March 2009. 7 May 2009 <http://www.sciencedaily.com­ /releases/2009/03/090331101105.htm>. • **Chisti, Yusuf. “Biodiesel from Microalgae.” Biotechnology Advances. Vol. 25, Pgs. 294-306. February 13, 2007. • CNRS. "Renewable Energies: The Promise Of Organic Solar Cells." ScienceDaily 10 April 2009. 7 May 2009 <http://www.sciencedaily.com­ /releases/2009/04/090409151444.htm>. • DOE/Los Alamos National Laboratory. "New Hope For Biomass Fuels: Breaking The Ties That Bind." ScienceDaily 29 April 2009. 3 May 2009 <http://www.sciencedaily.com­ /releases/2009/04/090422121904.htm>. • **Fouchard, Swanny. "Autotrophic and Mixotrophic Hydrogen Photoproduction in Sulfur Deprived C.Reinhardtii." Applied and Enviornmental Microbiology 71 (2005): 6199-6205. 16 May 2008 <http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1265920&blobtype=pdf> • **Kim Pyo, Jun; et al. “Enhancing hydrogen production by controlling light intensity in sulfur-deprived Chlamydomonas reinhardtii culture.” International Journal of Hydrogen Energy. Vol.31, Pgs. 1585-1590., September 2006. • **Najafpour, G. "Continuous Hydrogen Production via Fermentation of Synthesis Gas." Petroleum and Coal 45 (2003): 154-158. 12 May 2008 <http://www.vurup.sk/pc/vol45_2003/issue3-4/pdf/14.pdf>. • National Institute of Standards and Technology. "Discovery Of An Unexpected Boost For Solar Water-splitting Cells." ScienceDaily 26 April 2009. 3 May 2009 <http://www.sciencedaily.com­ /releases/2009/04/090423105853.htm>. • Natural Environment Research Council. "Plants Absorb More Carbon Dioxide Under Polluted Hazy Skies." ScienceDaily 23 April 2009. 23 April 2009 <http://www.sciencedaily.com­ /releases/2009/04/090422132829.htm>. • Weizmann Institute of Science. "New Way To Split Water Into Hydrogen And Oxygen Developed." ScienceDaily 8 April 2009. 23 April 2009 <http://www.sciencedaily.com­ /releases/2009/04/090406102555.htm>. • **(articles used from last years study)

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