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1. Article title: Dose- and Time-dependent Expression Patterns of Zebrafish Orthologs of Selected E2F Target Genes in Response to Serum Starvation/Replenishment Journal name: Molecular Biology Reports Author names: Ceren Sucularli · Serif Senturk · Mehmet Ozturk · Ozlen Konu Corresponding author: Ozlen Konu Department of Molecular Biology and Genetics, Faculty of Science, Bilkent University, 06800, Ankara, Turkey Tel: +90312 290 21 23 Fax: +90312 266 50 97 E-mail: konu@fen.bilkent.edu.tr

2. Fig. S1 Cell cycle analysis of ZF4 cells in response to serum fluctuations. 1.5·106 ZF4 cells were seeded in 100mm petri dishes and cultured in DMEM/F12 supplemented with 0.1%FBS or 10%FBS for 24 h. The cell cycle analysis was performed as explained in the materials and methods section. At each sampling, 1·104 cells were analyzed by Becton Dickinson FACScalibur. 24h 0.1% FBS 24h 10% FBS Propidium iodide Propidium iodide