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Tryptophan

Tryptophan. Cysteine is degraded by 2 pathways (1) Oxidation by cysteine dioxygenase. So…Cys = pyruvate + NH 3. If: Pyruvate = 4 NADH + GTP + FADH 2. Then: Cys = 4 NADH + GTP + FADH 2 + NH 3. If GTP = ATP NADH = 3 ATP FADH 2 = 2 ATP H 3 = -2 ATP. Then: Cys = 13 ATP. However….

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Tryptophan

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  1. Tryptophan

  2. Cysteine is degraded by 2 pathways (1) Oxidation by cysteine dioxygenase

  3. So…Cys = pyruvate + NH3 If: Pyruvate = 4 NADH + GTP + FADH2 Then: Cys = 4 NADH + GTP + FADH2 + NH3 If GTP = ATP NADH = 3 ATP FADH2 = 2 ATP H3 = -2 ATP Then: Cys = 13 ATP However…..

  4. (2) Cysteine desulfurylase: looks like serine dehydratase

  5. Cys = 1 pyruvate + NH3 + H2S

  6. Pyruvate + NH3 + ATP + NADPH ---> alanine + ADP + Pi + NADP+

  7. Methionine

  8. Acetohydroxyacid isomeroreductase

  9. Citrate synthase Aconitase IDH

  10. Pyruvate + -ketobutyrate + 2 NADPH + NH3 + ATP ---> Isoleucine + 2NADP+ + ADP + Pi 2Pyruvate + 2 NADPH + NH3 + ATP ---> valine + 2NADP+ + ADP + Pi 2Pyruvate + 2 NADPH + NH3 + ATP + Acetyl-CoA + NAD+ ---> leucine + 2NADP+ + NADH + ADP + Pi

  11. Degradation of branched chain amino acids Valine, leucine and isoleucine First three steps are shared Branched chain amino acid aminotransferase

  12. Leucine degradation

  13. So….Leucine = 3 Acetyl-CoA + NADH + FADH2 + NH3 - ATP If: succinyl-CoA goes to succinate then you lose a GTP Then: Leu = 3 Acetyl-CoA + NADH + FADH2 + NH3 - 2 ATP If: Acetyl-CoA = 3 NADH + FADH2 + GTP Then: Leu = 10 NADH + 4 FADH2 + 3 GTP + NH3 - 2 ATP If GTP = ATP, NADH = 3 ATP FADH2 = 2 ATP and NH3 = -2 ATP Then: Leu = 37 ATP

  14. Valine degradation

  15. So…propionyl-CoA = succinyl-CoA - ATP Then: Valine = 3 NADH + FADH2 + succinyl-CoA - ATP + NH3 If: Succinyl-CoA = malate + GTP + FADH2 If: GTP = ATP Then: Val = malate + 3 NADH + 2 FADH2 + NH3 If: Malate = pyruvate + NADPH - NADH Then: Val = 2 FADH2 + pyruvate + NADPH + 2 NADH + NH3 If: Pyruvate ---> 4 NADH + GTP + FADH2 Then: Val = 6 NADH + GTP + 3 FADH2 + NADPH + NH3 If GTP = ATP, NADH = 3 ATP FADH2 = 2 ATP and NH3 = -2 ATP Then: Val = 23 ATP + NADPH

  16. Isoleucine Degradation

  17. So…propionyl-CoA = succinyl-CoA - ATP Then: Isoleucine = 2 NADH + FADH2 + Acetyl-CoA + succinyl-CoA - ATP + NH3 If: Succinyl-CoA = malate + GTP + FADH2 If: GTP = ATP Then: Ile = Acetyl-CoA + malate + 2 NADH + 2 FADH2 + NH3 If: Malate = pyruvate + NADPH - NADH Then: Ile = Acetyl-CoA + 2 FADH2 + pyruvate + NADPH + NADH + NH3 If: Pyruvate ---> 4 NADH + GTP + FADH2 If: Acetyl-CoA ---> 3 NADH + GTP + FADH2 Then: Ile = 8 NADH + 2 GTP + 4 FADH2 + NADPH + NH3 If GTP = ATP, NADH = 3 ATP FADH2 = 2 ATP and NH3 = -2 ATP Then: Ile = 32 ATP + NADPH

  18. 3Glucose-6-P 3Ru5P + Gln F6P + E4P + R5P 3PG NH3 + 2PEP + E4P + R5P + Serine Tryptophan + pyruvate + GAP + CO2

  19. Aldolase

  20. 2-keto-3-deoxy-arabinoheptulosonate-7-phosphate synthase

  21. chorismate synthase

  22. Anthranilate Chorismate Prephenate p-hydroxybenzoate p-aminobenzoate Tryptophan Nictotinamide Phenylalanine Tyrosine Tetrahydrofolate Coenzyme Q

  23. +

  24. Ribose-5-phosphate Cost = 2 ATP

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