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La Cellule

La Cellule. DNA. Les nucléotides. Le code génétique. Chromosomes. Nucleosomes. Gene Expression. La Transcription. Epissage alternatif. Translation(traduction). La traduction. La réplication de l’ADN. Réplication de l’ADN. Procédure complète. 37°C. Polymerase Chain reaction. + Sonde.

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La Cellule

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  1. La Cellule

  2. DNA

  3. Les nucléotides

  4. Le code génétique

  5. Chromosomes

  6. Nucleosomes

  7. Gene Expression

  8. La Transcription

  9. Epissage alternatif

  10. Translation(traduction)

  11. La traduction

  12. La réplication de l’ADN

  13. Réplication de l’ADN

  14. Procédure complète 37°C

  15. Polymerase Chain reaction

  16. + Sonde • Hybridation sur colonie de bactéries, sur plage de lyse • Objectif: détecter parmi un grand nombre de bactéries ou de phages recombinants celle ou celui qui contient le fragment d'ADN recherché (criblage de banque). Boîtes avec colonies bact ou phages (milliers à millions) Coussin de NaOH (éclattement cellules + dénaturation ADN Hybridation Lavages Prise d'empreinte (nylon ou nitrocellulose) Autoradiographie Localisation des clones d'intérêt Fixation (cuisson ou UV)

  17. Criblage d’une banque phagique An Introduction to Genetic Analysis

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