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TESTING GENETICALLY MODIFIED ORGANISMS & LEGALITIES

TESTING GENETICALLY MODIFIED ORGANISMS & LEGALITIES. DR.K.S.MANJA. GMO- Plants created for food Desired traits Genes – CaMV 35S Promoter NOS Terminator Bar gene Pat gene 45% Soya 50% cotton 60% Super Market. U.K. -- Resist Regulatory mistakes Mistrust Gene flow

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TESTING GENETICALLY MODIFIED ORGANISMS & LEGALITIES

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  1. TESTINGGENETICALLY MODIFIED ORGANISMS & LEGALITIES DR.K.S.MANJA

  2. GMO- Plants created for food • Desired traits • Genes – CaMV 35S Promoter • NOS Terminator • Bar gene • Pat gene • 45% Soya • 50% cotton • 60% Super Market

  3. U.K. -- Resist • Regulatory mistakes • Mistrust • Gene flow • Threat to biodiversity • Allergens • Antibiotic resistance transfer • Labelling ?

  4. What to test for ? • Introduced DNA • Expressed product • Protein based product • Use western blot technique • Immunoassay • Sensitivity 1%

  5. ELISA • Highly sensitive (0.25%) • Limitation if protein not expressed • DNA - PCR/Southern Blot • 20 pg. to 10 ng. • Quantitative PCR • Real time PCR

  6. Non-destructive testing • Near infrared spectroscopy • Less than 1 min. • PCR – Protein • Both negative • Undetectable level

  7. Problems • Not all products have DNA • Oil / Denature • Societal resistance for GMO • Fear of unknown • Terminator Gene • Gene flow • Allergens • Brazil nut methionine

  8. LEGAL ASPECTS OF BIOTECHNOLOGY HAZRDOUS & GE MICROBES MIN.OF ENV & FORESTS, 1989 RECOMBINENT DNA SAFETY MIN OF S & T / DBT NATIONAL BT STRATEGY DBT / MIN. S & T FOOD SAFETY & STD. BILL MIN.OF FOOD PROSS. INDUSTRIES

  9. RULES FOR HAZARDOUS & GE MICROBES • SALE STORAGE AND HANDLING • IMPORT / EXPORT • PRODUCTION / PACKAGING • AUTHORITIES • RECOMBINANT DNA ADV COMMITTE • DBT • REVIEW COMMITTEE – GEN MANUP.

  10. DBT,ICMR, ICAR, CSIR • INSTITUTIONAL BIOSAFETY COMM. • GEN ENGG APPROVAL COMM. • DEPT ENV & FORESTS • STATE BT CO-ORD.COMM. • DIST.LEVEL COMM. • DC/PCB/CMO/AGRI OFFICER/MB • BACTERIA,FUNGI,PARASITES,VIRUS

  11. APPROVAL/PROHIBITION • NO WORK- HAZ / GE MICROBES • WITHOUT GEAC. APPROVAL • PERMITTED IN RES / MIN ENV FOREST • EDUCATION /I. BIO. SAFETY COMM • NO DELIBERATE RELEASE • SPECIC PERIOD (4 YEARS) • SUPERVISION.GEAC/SBCC/PCB/DLC

  12. PENALTIES • DLC / SBCC – USER EXPENCE • URGENCY /STATE ACTION / RECOVER • SEEK GOVT-IMPLEMENTATION • ACCIDENTS TO BE NOTIFIED. • APPEAL NEXT AUTHORITY • 30 DAYS • POLICE OR FOREST DEPT HELP

  13. RISK GROUP • II ORD BACTERIAL PATHOGENS • III Cl botulinum/Plague. • II.FUNGI. CRYPTOCOCCUS • III HISTOPLASMA CAP. • II VIRUSES. HEPATITIS. DENGUE. • III MONKEY POX / RABIES • IV KFD / RSSE • SPECIAL-LASSA / S.POX / YF / MARBERG

  14. PLANTS • ALL VIRUSES • BACTERIA – XANTHOMONAS • FUNGI - 50 FAMILIES • PARASITIC WEEDS • PROTOZA. PHYTOMONAS • NEMATODES. 23 FAMILIES. • ARTHROPOD PESTS

  15. R. DNA SAFETY REGULATION • DBT. GE ORGANISMS • G. TRA. GREEN PLANTS / ANIMALS • R-DNA TECH – VACCINES • LARGE SCALE PDN PROHIBITED • EXEMPT(SELF CLONING) • COMPETENT AUTH(NON PATH) • APPROVAL(TOXIN GENE / DRUG RES)

  16. CONTAINMENT-LEVAL 3 - 4 • GOOD LAB PRACTICE • BIOLOGICAL CONTAINMENT • VECTOR SPECIFIC • SUVIVAL IN ENVIRONMENT • GREEN HOUSE CONTAINMENT • NEGATIVE PRESSURE • POSITIVE PRESSURE

  17. GREEN HOUSE • NON PATHOGEN VECTOR GH (A) • PATHOGEN VECTOR GH (B) • 20Lit AND ABOVE – LARGE SCALE • GE ORG SHOULD NOT BE PATHOGEN • RELEASE TO ENVIRONMENT • GENETIC MARKERS / HOST RANGE • STABILITY FOR 4 YEARS

  18. BIOWASTES TO BE DESTROYED • RESEARCH – DEPT.BIOTECH. • CGO COMPLEX NEW DELHI 110003 • ENV RELEASE – MIN ENV & FORESTS • PARYAVARAN BN. NEW DELHI.110003 • SUPPORTING ORGGANISATIONS • I C M R (AIIMS COMPLEX) • I C A R (KRISHI BN ) • C S I R (RAFI MARG)

  19. BIOHAZARD • GREEN HOUSE – A • TRAINING BIOHAZARD • I B S C • GREEN HOUSE - B • NEGATIVE PRESSURE • DOUBLE DOOR - POLLEN CONTROL • EFFLUENT TREATMENT

  20. EMPOWERING JUDICIARY • TRAING FOR JUDICIARY • CENTRE FOR FINGER PRINTING • TRG CENTRE FOR BIO – SAFETY • NATIONAL BIOTECH. AWARENESS FUND • HUMAN TISSUE ACT 2005 • IPR IN COLLEGE CURRICULUM • PUBLIC PARTICIPATION

  21. MULTIPLICITY OF FOOD LAWS • GROUP OF MINISTERS • INTEGRATED FOOD LAWS • FOOD SAFETY & STANDARD ACT 2005 • GENETICALLY MODIFIED FOODS • LABELLING OF FOOD PRODUCTS • CODEX ALIMENTARIOUS • P F A ACT. LABEL. WTS & MEASURES

  22. FOOD AUTHORITY NEW DELHI • AGRICULTURE / COMMERCE / HEALTH • FOOD PROC / LAW / A FOOD TECH • SCIENTIFIC COMMITTEE • FRADULENT PRACTICE.ADULTERATION • GM FOODS • STATE COMMISSIONER FOR F. SAFETY • SPCIAL APPELLATE TRIBUNAL • SPECIAL COURT (GAS TRAGEDY)

  23. Thank you

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