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(1) When hydrogen peroxide decomposes it gives off oxygen gas. The rate is

(1) Describe how the apparatus shown below is used to measure the rate of the enzyme catalysed decomposition of hydrogen peroxide. Side arm tube. Note:- the potato discs contain the enzyme catalase. Potato discs. Hydrogen peroxide.

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(1) When hydrogen peroxide decomposes it gives off oxygen gas. The rate is

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  1. (1) Describe how the apparatus shown below is used to measure the rate of the enzyme catalysed decomposition of hydrogen peroxide. Side arm tube Note:- the potato discs contain the enzyme catalase. Potato discs Hydrogen peroxide The effect of changes in pH and temperature on the decomposition of hydrogen peroxide (Unit 2 PPA 3) The aim of the experiment is to show the effect of changing temperature or pH on the enzyme catalysed decomposition of hydrogen peroxide. (1) When hydrogen peroxide decomposes it gives off oxygen gas. The rate is determined by counting the number of bubbles of gas given off in 1 minute. (2) Buffer solutions of differing pH values (4, 7 and 10) are added to the hydrogen peroxide solution. (2) How can the effect of pH be investigated? (3) The hydrogen peroxide solution is warmed by placing the side arm tube in a beaker of hot water until the desired temperature is reached. Then the potato discs are added. (3) How can the effect of temperature be investigated?

  2. Number of bubbles of gas 20 15 10 5 Temperature (oC) (4) The graph shows the effect of temperature on the number of bubbles of oxygen gas given off in 1 minute. Describe how the activity of the enzyme is affected by changes in temperature. (4) The enzyme’s activity is highest at a temperature of about 37oC. At temperatures higher, or lower than this value the activity of the enzyme decreases sharply.

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