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CWRS Advisory Board Meeting March 1, 2007 Gretchen Grover – Chemist Christal Wade - Microbiologist Rick Fowler – Environ

CWRS Advisory Board Meeting March 1, 2007 Gretchen Grover – Chemist Christal Wade - Microbiologist Rick Fowler – Environmental Biotechnologist. WATERS Employees. Director: Jana Fattic Lab Analysts: Microbiology Department: Rick Fowler, Christal Wade

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CWRS Advisory Board Meeting March 1, 2007 Gretchen Grover – Chemist Christal Wade - Microbiologist Rick Fowler – Environ

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  1. CWRS Advisory Board Meeting March 1, 2007 Gretchen Grover – Chemist Christal Wade - Microbiologist Rick Fowler – Environmental Biotechnologist

  2. WATERS Employees • Director: Jana Fattic • Lab Analysts: • Microbiology Department: Rick Fowler, Christal Wade • Chemistry Department: Gretchen Grover, Dong Li • Office Associate: Kristy Mayes • Students: Samantha Kramer, Rebekkah Lively, Jennifer Dean

  3. Customers • Private • Total Coliform/E. Coli, Metals, Ions, Atrazine • Wastewater • BOD, pH, TRC, DO, TSS, Fecal Coliform, Ammonia Nitrogen • Storm water • COD, Oil and Grease, TDS, TSS • Drinking water • Total Coliform/E.Coli • Oil Wells • pH, TDS, Specific Gravity, Chloride • Research Projects • TOC, Atrazine, Metals, Ions, DNA

  4. Certifications • Drinking water Certification • January Audit • Inorganic Certification • June Audit • Cryptosporidium • Long Term 2 Enhanced Surface Water Treatment Rule (LT2) • Accept outside samples under LT2 and provides field filtration apparatus • www.epa.gov/safewater/disinfection/LT2/lab_aprvlabs.html • Chemical Hygiene Plan • Chemical Hygiene Officer

  5. Projects • Mammoth Cave • Flood Pulse Event • City of Bowling Green • River & Cave System monitoring • Watershed Watch • Upper Green River watershed monitoring (E.coli) • TMDL • Analyzing samples for this project • University Research • Support WKU faculty as well as other university projects

  6. Quality Assurance • Continued revision and updating of SOP to the 21st Edition Standard Methods • QA/QC Improvements • New Standards • PT/QC (quarterly) • USGS • APG

  7. Chemistry Accumet excel XL60 TKN Digestor Konelab Microbiology Bench top water bath FastGel electrophoresis apparatus PCR Workstation Qubit fluorometer Microcentrifuge Important Purchases

  8. Where do we go from here? • Current Capabilities • Cryptosporidium • Konelab Analyses • Drinking water, Wastewater, Storm water analyses • Bacteriological/DNA analyses • Future Capabilities • Unregulated Contaminant Monitoring Rule (UCMR2) • Organics (Work toward a GC MS/MS and HPLC) • Radionuclides • Direct a Chemical Laboratory Certification Program for Kentucky • Voluntary Wastewater Certification

  9. Where do we go from here? • New customers bring more cash flow • 11 new customers • Networking; customer surveys • Pick up service • Consultation services • Sample collection services

  10. Microbiology Programs Christal Wade

  11. Drinking Water Microbiology Completed latest On-Site Audit in January 2007

  12. EPA Approval for Crypto • WATERS Lab received approval from EPA for the analysis of Cryptosporidium on October 18, 2006. • Currently have a cooperative agreement with Appalachian States Analytical, L.L.C. • Total of 20 surface water clients • Will be starting monitoring in June • 4 Contracts with other PWSs • From both Kentucky and West Virginia • Several quotes outstanding

  13. Latest PT ResultsFrom September 2006 Completed another set of PT samples in February 2007

  14. Ongoing Precision and Recovery Mean Recovery 52.4%

  15. Ongoing Precision and Recovery Mean Recovery 41.1%

  16. Education & Outreach • 2 Hour Crypto Presentation • Approved for Drinking Water Operator CEU • Presented at KRWA Training Sessions • Will Present at KWWOA Annual Conference • 1 Hour E. coli Presentation • Approved for Waste Water Operator CEU • Presented at KRWA Training Sessions • Will Present for KWWOA

  17. Environmental Biotechnology RickFowler

  18. DNA Analysis Services • DNA extraction from water, soil, membrane filters, pure cultures, swabs, etc. • Yield of total DNA measured with Qubit fluorometer, sensitivity 0.2 x 10-12 g • Quantitative Real-Time PCR • Determines concentration of DNA from specific organisms in a mixture, sensitivity <1 ppt • DNA cloning and sequencing • Cloning permits segregation of individual species comprising environmental microbial communities • DNA sequencing yields unique gene sequences for identification of organisms

  19. Fungi Parasites Bacteria Amoebae Algae Zooplankton

  20. Field work in Mammoth Cave Limestone Ca13CO3

  21. Examples of clients supported by WATERS DNA services • Louisville Water Company • Ammonia Oxidizing Bacteria in distribution system • Acanthamoeba in eyewash stations • KSU Aquaculture Research Center • DNA analysis of bacteria, algae, and fungi used to evaluate conditions for optimal shrimp production • Fungi in some tanks identified by cloning and DNA sequencing to assess potential risk or benefit • National Cave and Karst Research Institute • Develop a universal monitoring scheme applicable to caves nationwide and catalog any new microorganisms indigenous to cave climates

  22. Examples, continued • Mammoth Cave National Park • Ongoing monitoring of bacterial communities for better understanding of karst aquifers and as indicators of environmental changes • Monitor Chlorella DNA in surface waters to assist with protection of endangered mussells • Oregon Caves National Monument • Cloning and sequencing of bacterial and fungal communities on- and off-tourist trails to assess environmental impacts of tourism • Abundance of iron-sulfur bacteria indicate growth in absence of light is derived from chemical energy

  23. Scientific Outreach, Presentations, and Workshops • National Speleological Society • 2001, 2006 • National Cave and Karst Research Institute • 2001, 2005 • Geological Society of America • 2001, 2004 • National Park Service advisory panels • 2001, 2002, 2003, 2004, 2005, 2006 • ISEIS • 2006

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