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DNA variation in Ecology and Evolution III- Examples of application of molecular markers

Maria Eugenia D’Amato. DNA variation in Ecology and Evolution III- Examples of application of molecular markers. BCB 703: Scientific Methodology. Gene flow and phylogeography Agama atra in Southern Africa. Phylogeographic reconstruction: Cytochrome b (540 bp) 16S rDNA (476 bp).

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DNA variation in Ecology and Evolution III- Examples of application of molecular markers

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  1. Maria Eugenia D’Amato DNA variation in Ecology and EvolutionIII- Examples of application of molecular markers BCB 703: Scientific Methodology

  2. Gene flow and phylogeographyAgama atra in Southern Africa Phylogeographic reconstruction: Cytochrome b (540 bp) 16S rDNA (476 bp)

  3. Phylogeography of Agama atra MP tree ML tree

  4. Phylogeography of Agama atraCoincident pattern with other rock-dwelling species Pronolagus rupestris Pachydactilus rugosus Vicariant event cycles of dry-humid period during glacial –interglacial produced fragmentation of habitat

  5. 2.a. Gene flow between species: the problem of hybridsTilapias O. niloticus O. aureus Hybrids in the wild?

  6. Tilapias hybridization Cluster of population Nuclear Mitochondrial O.aureus * Nile Niger Cluster of haplotypes Senegal * Haplotypes common to both species

  7. Tilapias hybridization • Main results • shallow mtDNA divergence between species in Sudano- Sahelian zone • large divergence between Nile- western Africa Retention of ancestral polymorphisms? Secondary contact + introgression? hypotheses

  8. 2.b. Tilapias hybridization in South Africa O. mortimeri O. karongae O. andersonii Parsimony network of mtDNA control region O. mossambicus

  9. 3. Phylogeny of Abalone with nuclear sequences Haliotidae Fissurellidae Orthologs ~ 65% identity 260 MY 350 MY paralogs Duplication event

  10. Phylogeny of abalones 65 % identity orthologs 80-95 % identity orthologs paralogs

  11. 4. Population structure- introduced forms Chinese threeline grunt Japanese samples Population structure analysis with 4 microsatellites loci significant

  12. Population structure in the threeline Chinese grunt Pairwise Fst between populations significant

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