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Introduction to the Compound Light Microscope

Introduction to the Compound Light Microscope. Chuck Hesbacker April 2010. Introduction to the Compound Light Microscope. Objectives:. Label the parts of the CLM Learn the function of the parts of the CLM Operate the CLM CLM = Compound Light Microscope.

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Introduction to the Compound Light Microscope

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  1. Introduction to the Compound Light Microscope Chuck Hesbacker April 2010

  2. Introduction to the Compound Light Microscope Objectives: • Label the parts of the CLM • Learn the function of the parts of the CLM • Operate the CLM CLM = Compound Light Microscope

  3. Introduction to the Compound Light Microscope Warm up exercise: Write down at least 5 things you know about the microscope in your class journals.

  4. Some Useful Facts About Who Invented The Microscope Introduction to the Compound Light Microscope • In 1590, Zaccharias Janssen and his father Hans invented the first microscope. • Galileo added a focusing device. • Anthony Leeuwenhoek was called the “Father of Microscopy” because he could obtain magnifications of 270X. • Leeuwenhoek was the first to see bacteria, yeast, and blood cells. • Robert Hooke was called the “English Father of the Microscope” because he made improvements to the microscope.

  5. Introduction to the Compound Light Microscope Today we will Label the parts of the CLM Learn the function of the parts of the CLM Operate a CLM CLM = Compound Light Microscope

  6. Introduction to the Compound Light Microscope Handout: Diagram of the Microscope Don’t label the diagram yet!

  7. Fill out the blanks as we proceed through the lesson. Introduction to the Compound Light Microscope

  8. The body tubeseparates the objective and the eyepiece. Copy this into your lab manual. Introduction to the Compound Light Microscope Body Tube

  9. The revolving nosepiece holds two or more objective lenses and can be rotated to change power. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece

  10. The 10x objective magnifies the image 10 times. It is the lowest power objective. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece 10x Objective

  11. The 43x objective magnifies the image 43 times. It is the medium power objective. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece 10x Objective 43x Objective

  12. The 97x objective magnifies the image 97 times. It is the high power objective and requires oil immersion. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece 10x Objective 43x Objective 97x Objective

  13. The stage clips hold the slides into position and prevent sliding. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece 10x Objective 43x Objective 97x Objective Stage Clips

  14. The diaphragm controls the amount of light that passes through the object being viewed. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece 10x Objective 43x Objective 97x Objective Stage Clips Diaphragm

  15. The illuminator is a steady light source that illuminates the slide. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece 10x Objective 43x Objective 97x Objective Stage Clips Diaphragm Illuminator

  16. Eyepiece Lens The eyepiece lens is the lens at the top that you look through.  They are usually 10X or 15X power.  Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece 10x Objective 43x Objective 97x Objective Stage Clips Diaphragm Illuminator

  17. Eyepiece Lens The arm supports the tube and connects it to the base. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece The Arm 10x Objective 43x Objective 97x Objective Stage Clips Diaphragm Illuminator

  18. Eyepiece Lens The stage is the flat platform where you place your slides. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece The Arm 10x Objective 43x Objective The Stage 97x Objective Stage Clips Diaphragm Illuminator

  19. Eyepiece Lens The course focus is the larger knob that is first used to focus because it makes large adjustments to the focus. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece The Arm 10x Objective 43x Objective The Stage 97x Objective Stage Clips Coarse Focus Diaphragm Illuminator

  20. Eyepiece Lens The fine focus is the smaller knob that makes small adjustments to the focus. Use the coarse focus first and then use the fine focus. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece The Arm 10x Objective 43x Objective The Stage 97x Objective Stage Clips Coarse Focus Diaphragm Fine Focus Illuminator

  21. Eyepiece Lens The base supports the microscope. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece The Arm 10x Objective 43x Objective The Stage 97x Objective Stage Clips Coarse Focus Diaphragm Fine Focus Illuminator The Base

  22. “using the compound Microscope” Introduction to the Compound Light Microscope

  23. Eyepiece Lens • Go to your laboratory station and setup your microscope. • Setup slides on microscopes. • Examine the 8 slide set and identify in book. Introduction to the Compound Light Microscope Body Tube Revolving Nosepiece The Arm 10x Objective 43x Objective The Stage 97x Objective Stage Clips Coarse Focus Diaphragm Fine Focus Illuminator The Base

  24. Return to your desk and label the parts of the microscope for a grade. Introduction to the Compound Light Microscope

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