1 / 4

E. Coli response to Theophylline and Caffeine

E. Coli response to Theophylline and Caffeine. Procedure.

jadyn
Download Presentation

E. Coli response to Theophylline and Caffeine

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. E. Coli response to Theophylline and Caffeine

  2. Procedure Using a plate reader absorbance was measured of J11940+J119303, J119140+J119304, J11940 in pSB4c5, J119140 in pSB1a2, K315009, and J100111 in the presence of 1 mM of theophylline and separately in the presence of 5 mM of caffeine. Each combination was tested in the presence of 10 microgram/mL of tetracycline (low concentration), 20 microgram/mL oftetracycline, and no tetracycline. Absorbance was measured every hour for 23 hours

  3. Results 15 hour absorbance minus initial absorbance with 1 mM Theophylline All cells except J100111 grew in the presence of Theophylline in all Tetracycline concentrations. It would be expected that the theophylline would have allowed transcription of the Tetracycline resistance in J100111 allowing it to grow in high Tetracycline. Perhaps not enough Tetracycline resistance was made to successfully pump out the high concentration of Tetracycline.

  4. Results 15 hour absorbance minus initial absorbance with 5 mM Caffeine All cells grew in the presence of Caffeine whenever no Tetracycline was present. This shows that the Caffeine is not killing the cells. However, in the presence of Tetracycline only K315009 (the control) grew successfully. These results show that the tetracycline resistance is not being expressed in the other cells.

More Related