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DISCOVERY OF GIBBERELLINS. “BAKANAE” OR “FOOLISH SEEDLING” DISEASE OF RICE : plants grew too talkk and toppled over. JAPANESE SCIENTISTS WORKED ON THE PROBLEM AND IDENTIFIED CAUSE: INFECTION BY FUNGUS, GIBBERELLA FUJUKUROI.
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DISCOVERY OF GIBBERELLINS “BAKANAE” OR “FOOLISH SEEDLING” DISEASE OF RICE : plants grew too talkk and toppled over. JAPANESE SCIENTISTS WORKED ON THE PROBLEM AND IDENTIFIED CAUSE: INFECTION BY FUNGUS, GIBBERELLA FUJUKUROI. MID 1950s: ACTIVE SUBSTANCE CRYTALIZED AND IDENTIFIED IN ENGLAND AND US: GIBBERELLIC ACID (GA3)
Rice leaf sheath bioassay for gibberellin Increasing GA3 concentration
GIBBERELLIN BIOSYNTHESIS AND METABOLISM GIBBERELLINS ARE SYNTHESIZED VIA THE TERPENOID PATHWAY. BASED ON ISOPRENE UNITS: BIOLOGICAL ISOPRENE UNIT = ISOPENTENYL DIPHOSPHATE
LOCATIONS OF SOME MUTANT GENES THAT BLOCK GA BIOSYNTHETIC PATHWAY IN PEA
GA1, THE BIOLOGICALLY ACTIVE GIBBERELLIN, IS SYNTHESIZED FROM GA20
Phenotypes and genotypes of peas with different gibberellin levels
Luciferase reporter construct for GA 20-oxidase HIGHEST IN APICAL BUD
Effect of Long Days (High GA levels) on Tuber Formation in Potato Long Days Short Days
Genetically Engineered Dwarf Wheat Plants Overexpressing GA 2-ox, which degrades GA1
KINETICS OF GA-INDUCED ELNGATION OF DEEPWATER RICE INTERNODE 30 min
DISTRIBUTION OF CELL DIVISION FOLLOWING GA TREATMENT OF A ROSETTE PLANT, Samolus parviflorus
Arabidopsis GIBBERELLIN RESPONSE MUTANTS WITH DEFECTS IN SIGNAL TRANSDUCTION 1. Gibberellin-in sensitive dwarfs 2. Gibberellin-deficient mutants in which GA deficiency has been overcome by a second suppressor mutation, so plant looks normal (double mutant) 3. Mutants with constitutive gibberellin response (“slender” mutants)
GAI and RGA are related repressor proteins Repression of GA Response Genes GA Response Genes Expressed
DIFFERENT TYPES OF MUTATIONS OF GAI AND RGA REPRESSORS AFFECT GROWTH DIFFERENTLY
GA acts to block action of both SPY and GAI/RGA Enzyme involved in protein modification - enhances activity of GAI and RGA
EFFECT OF GA ON CALCIUM IN BARLEY ALEURONE LAYER PROTOPLAST CONTROL + GA3 GA3 + ABA (ABSCISIC ACID) Low Cytoplasmic [Ca2+] High