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DISCOVERY OF GIBBERELLINS

DISCOVERY OF GIBBERELLINS. “BAKANAE” OR “FOOLISH SEEDLING” DISEASE OF RICE : plants grew too talkk and toppled over. JAPANESE SCIENTISTS WORKED ON THE PROBLEM AND IDENTIFIED CAUSE: INFECTION BY FUNGUS, GIBBERELLA FUJUKUROI.

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DISCOVERY OF GIBBERELLINS

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  1. DISCOVERY OF GIBBERELLINS “BAKANAE” OR “FOOLISH SEEDLING” DISEASE OF RICE : plants grew too talkk and toppled over. JAPANESE SCIENTISTS WORKED ON THE PROBLEM AND IDENTIFIED CAUSE: INFECTION BY FUNGUS, GIBBERELLA FUJUKUROI. MID 1950s: ACTIVE SUBSTANCE CRYTALIZED AND IDENTIFIED IN ENGLAND AND US: GIBBERELLIC ACID (GA3)

  2. Rice leaf sheath bioassay for gibberellin Increasing GA3 concentration

  3. GIBBERELLIN BIOSYNTHESIS AND METABOLISM GIBBERELLINS ARE SYNTHESIZED VIA THE TERPENOID PATHWAY. BASED ON ISOPRENE UNITS: BIOLOGICAL ISOPRENE UNIT = ISOPENTENYL DIPHOSPHATE

  4. LOCATIONS OF SOME MUTANT GENES THAT BLOCK GA BIOSYNTHETIC PATHWAY IN PEA

  5. GA1, THE BIOLOGICALLY ACTIVE GIBBERELLIN, IS SYNTHESIZED FROM GA20

  6. GA1 LEVELS CORRELATE WITH INTERNODE LENGTH

  7. Phenotypes and genotypes of peas with different gibberellin levels

  8. Luciferase reporter construct for GA 20-oxidase HIGHEST IN APICAL BUD

  9. EFFECT OF AMO-1618 ON PETIOLE ELONGATION OF SPINACH

  10. EFFECT OF LONG DAYS ON GA LEVELS IN SPINACH

  11. Effect of Long Days (High GA levels) on Tuber Formation in Potato Long Days Short Days

  12. Genetically Engineered Dwarf Wheat Plants Overexpressing GA 2-ox, which degrades GA1

  13. KINETICS OF GA-INDUCED ELNGATION OF DEEPWATER RICE INTERNODE 30 min

  14. DISTRIBUTION OF CELL DIVISION FOLLOWING GA TREATMENT OF A ROSETTE PLANT, Samolus parviflorus

  15. Arabidopsis GIBBERELLIN RESPONSE MUTANTS WITH DEFECTS IN SIGNAL TRANSDUCTION 1. Gibberellin-in sensitive dwarfs 2. Gibberellin-deficient mutants in which GA deficiency has been overcome by a second suppressor mutation, so plant looks normal (double mutant) 3. Mutants with constitutive gibberellin response (“slender” mutants)

  16. GAI and RGA are related repressor proteins Repression of GA Response Genes GA Response Genes Expressed

  17. DIFFERENT TYPES OF MUTATIONS OF GAI AND RGA REPRESSORS AFFECT GROWTH DIFFERENTLY

  18. GA acts to block action of both SPY and GAI/RGA Enzyme involved in protein modification - enhances activity of GAI and RGA

  19. THE CEREAL ALEURONE LAYER

  20. EFFECT OF GA ON CALCIUM IN BARLEY ALEURONE LAYER PROTOPLAST CONTROL + GA3 GA3 + ABA (ABSCISIC ACID) Low Cytoplasmic [Ca2+] High

  21. SUMMARY OF STEPS IN GA SIGNAL TRANSDUCTION PATHWAY

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