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Effects of fetal exposure to isoflurane on postnatal memory and learning in rats

Effects of fetal exposure to isoflurane on postnatal memory and learning in rats Huafeng Wei, MD, PhD Assistant Professor Department of Anesthesiology and Critical Care University of Pennsylvania. Financial Disclosure. Grant Support: March of Dimes Birth Defect Foundation

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Effects of fetal exposure to isoflurane on postnatal memory and learning in rats

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  1. Effects of fetal exposure to isoflurane on postnatal memory and learning in rats Huafeng Wei, MD, PhD Assistant Professor Department of Anesthesiology and Critical Care University of Pennsylvania

  2. Financial Disclosure Grant Support: March of Dimes Birth Defect Foundation ( #12-FY05-62) NIH NIGMS K08 073224-02 Department of Anesthesiology and Critical Care at University of Pennsylvania E+D fund

  3. Is Isoflurane Neurotoxic to Fetus Developing Brain? • Isoflurane induced apoptosis dose-and time-dependently in different neuronal cultures. • Isoflurane induced apoptosis in the developing brains of 7-day-old rat pups and subsequent memory and learning disability. • Isoflurane caused permanent memory loss, learning disability in aged rats. • Hypothesis: Isoflurane inhaled to pregnant mothers induced apoptosis in fetus developing brain and postnatal memory and learning disability.

  4. 1.3% Isoflurane Inhibited Apoptosis in Fetus Hippocampus CA1 and Retrosplenial Cortex Hippocampus CA1 Retrosplenial Cortex Caspase-3 Density

  5. Isoflurane Improved Spatial Reference Memory In Juvenile But Not Adult Rats Probe Test, * Means P<0.05

  6. Summary • 1.3% isoflurane for 6 hour in pregnant rats inhibited spontaneous apoptosis in fetus brain at 2 hour, but not at 18 hour or postnatal day 5 after treatment. • 1.3% isoflurane for 6 hour in pregnant rats improved spatial reference memory in postnatal juvenile rats (P36), but not in adult rats (P119). • 1.3% isoflurane for 6 hour in pregnant rats did not affect learning in postnatal rats.

  7. Acknowledgement University of Pennsylvania Grace Liang, Yujuan Li, Shouping Wang,Qiujun Wang, Wenlin Wei, Baobin Kang, Qing Cheng Meng Roderic Eckenhoff, Randy Pittman, Maryellen Eckenhoff

  8. The Morris Water Maze: A sensitive test of rodent memory, learning and behavior. Cued test (Swimming ability, P28) Place test (Learning and memory, P32, P115) Probe test (Memory, P36, P120)

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