Journey of a New Probiotic from Lab to Life: Research, Innovation, and Market Adoption

1. Life of new probiotic-from laboratory to beneficiary dr Ivana Strahinic, vice director of IMGGE

2. 1986 Affiliated Centre of International Centre for Genetic Engineering and Biotechnology (ICGEB) Seeking fundamental knowledge: Develop top-quality expertise and translate knowledge into practice use apply to improve the quality of life…

3. MOLECULAR GENETICS AND BIOMEDICINE EDUCATION FUNDAMENTAL RESEARCH BIOTECHNOLOGY human molecular genetics plant molecular genetics molecular genetics of microorganisms 2016-2026 SERVICES INNOVATIONS 2015 Staff 120 become an integral part of all IMGGE activities

4. Sector for LINK BETWEEN IMGGE RESEARCH AND INDUSTRY R&D Technology Transfer Market Research Results Marketable IP DEVELOPMENT OF IP INDUSTRY/MARKET CONTRACT RESEARCH CONTRACT RESEARCH IMGGE LICENSING NEW COMPANY CREATION NEW COMPANY CREATION

5. THE LIFE OF NEW PROBIOTIC from laboratory to beneficiary RESEARCH DISCOVERY DEVELOPMENT PATENTNIG PRODUCTION MARKETING ADOPTION Long-term and uncertain road

6. RESEARCH LACTIC ACID BACTERIA • 30 years of collection, identification, biochemical and genetic characterization • over 10 000 strains of LAB in LMM collection • searching of new bacteria • for treatments of disorders and diseases • replacement of antibiotic therapies • biomarkers for health and diseases • microbiome members contribute to health • source of new drugs • starters for dairy industry FUNDAMENTAL RESEARCH IMGGE GOAL – TO FIND A STRAINS WITH EXCELLENT PROBIOTIC FEATURES 0 1 0 2 7 8 9 1

7. DISCOVERY LACTIC ACID BACTERIA AS potential HUMAN and ANIMAL PROBIOTICS In vitrotesting - resistance to artificial gastric and intestinal fluids Ex vivo testing – gut tissue sections and Caco2, HT29MTX Antimicrobial testing In vivotesting – rat model of Crohn’s disease Lactobacillus sp. Lactobacillus sp. Streptococcus sp. FUNDAMENTAL RESEARCH + innovation project with veterinary station KOKER GOAL – Select THE BEST STRAINS that could be use AS PROBIOTICS Especially as ALTERNATIVE TO ANTIBIOTICS IN TREATMENT OF INTESTINAL INFECTIONS Additionally– strains possesses industrially important features for yogurt production 2 1 0 2 0 1 0 2

8. DEVELOPMENT 2 TYPES OF PRODUCTS PROBIOTIC FOR ANIMALS PROBIOTIC for HUMANS LIOPHYLIZED POWDER Phytonet - interested to commercialize through LICENSE agreement FERMENTED LIQUID PRODUCT for animal consumption Inventor open spin-out INVETLAB - interested to commercialize through LICENSE agreement • FUNDAMENTAL RESEARCH + innovation project with consortium • Veterinary station KOKER • Spin-out INVETLAB • Phytonet continuous help of the WORLD BANK EXPERTS 8 1 0 2 2 1 0 2

9. PATENTING 2 DIRECTIONS NATIONAL PATENT AND PCT related to application of probiotic DEPOSITION OF STRAINS UNDER PATENT DEPOSIT “NEW PROBIOTIC STARTER CULTURE FOR HUMAN AND ANIMAL APPLICATION“ Belgian coordinated collections of microorganisms NATIONAL PATENT: 2016. PCT: 2018. STT-IMGGE WORLD BANK EXPERTS Intellectual property office PETOSEVIC In parallel License agreement with Phytonet for human and animal use -world wide (except Balkan) License agreement with INVETLAB for animal use for Balkan 8 1 0 2 3 1 0 2

10. PRODUCTION 2 DIRECTIONS PROBIOTIC CAPSULE FOR HUMAN CONSUMTION FERMENTED DAIRY PRODUCT FOR ANIMAL USE First sale – during 2018. Current status – product formulation 0 2 0 2 8 1 0 2

11. MARKETING and ADOPTION PRESENTATION on global nutraceuticals event VITAFOODS 2019, GENEVA, Switzerland Natural and safe Eliminates hardly curable pathogens Improves general animal health Suitable for pregnant animals Bursts immunity in newborns

12. 1 national and 1 international patent what we achieved so far 2 different type of products for human and animal use 2 License agreements signed Spin-out company INVETLAB established how to round off the process of technology transfer frominvention to the innovation how to attract companies and negotiate with them how to write a good license agreement what we learned future stories through learned example easier to the new innovations

13. Sector for Microorganisms as probiotics • Phytonet, Switzerland • Medico Domus, Serbia Biomedicine • BluestonePharmA, Switzerland • Immunex, Turkey • VS Koker, Serbia • STEM ART d.o.o., Serbia • Invetlab doo, Adaševci, Serbia • Educell cell therapy service, Ltd., Slovenia • Surgical Center Toš ,Ltd., Ptuj, Slovenia Microorganisms in agriculture • Complete Genomics Inc., USA • Agrounik doo, Šimanovci • Phytonet doo, Beograd • Biofor Systems doo, Beograd Genetic test for women’s reproductive health improvement Development of NGS libraries • Supstrati Maki D.O.O, Grgurevci, Serbia Closed system for isolation of Bone MarrowMultipotent Stromal Cells Innovative portals for diseases Multifunctional microbial solution for healthy soil Products for the treatment of plant pathogens SensORing molecular sensor for malignancy screening Compoundsfor thetopicalapplicationintheskinprotectionagainst UV light Innovative technological process for improved production of vermicompost Microorganisms in food production Molecular methods • CSK Food Enrichment, Netherlands ZEOBION innovative product for DNA isolation • Invetlab doo, Adaševci, Serbia • Acies Bio, Slovenia Development of starter cultures for cheese and yoghurt production

14. THANK YOU FOR YOUR ATTENTION strahi@imgge.bg.ac.rs