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Development of a Murine Perfusion System. Nicola Asgill Fredrick Hilliard George Kittos. Objectives. Langendorff perfusion system Requirements: Miniature scale Fluid temperature controlled Inter-changeable components Drug introduction into perfusate. Background.
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Development of a Murine Perfusion System Nicola Asgill Fredrick Hilliard George Kittos
Objectives • Langendorff perfusion system • Requirements: • Miniature scale • Fluid temperature controlled • Inter-changeable components • Drug introduction into perfusate
Background • Langendorff Perfusion System • Simulates natural blood flow • Retrograde fluid flow • Attached to aorta • Examine effects of drug on heart
Considerations for project • Why will this work? • Controlled temperature, flow rate and pressure • Improvement of heat stability • Multi-drug delivery capability • Disposable components • Why is this important? • Possible testing applications: • Drug delivery • Cardiac cell response
Prototype Modifications for Preliminary Testing • Flow will be gravity controlled • Removal of Peristaltic Pump • Removal of Flow Sensor • Pump and Sensor will be added based on results of preliminary testing
Peltier Element Test • Goals • Show functionality – Heating capabilities • Calculate Time Constant • To heat from room temperature to 37 ± 1°C • Determine appropriate input voltage
Peltier Element Test • Conclusion: • Input Voltage of 8V – longer time to heat sample, needs to be faster to perfusate at constant temp. • Input Voltage of 15V – faster heating, but found that this voltage will cause the device to fail. • Input Voltage of 12V – faster heating and the device did not malfunction
Transparent Glass Chamber • Plexiglass • Visualize electric activity in heart using dyes • Staggered holes
Tygon Tubing Peltier Element Current Work • Heating Perfusate to 37 ± 1°C • Manufacturing Plexiglass chamber • Verify and Validate the LabView Module for controlling and maintaining constant temperature
Future Work • Acquiring additional components • Assembling Prototype • Testing the perfusate heating apparatus • Design and Test Control Valve System • Harvesting the Mouse Heart
Thanks Questions?