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SIMULATION OF A BIOREACTOR Tiffany Tarrant Todd Giorgio

SIMULATION OF A BIOREACTOR Tiffany Tarrant Todd Giorgio. What is a Bioreactor?. Experimental device used to culture cells Provides nutrient media, oxygen support, fluid environment, area to grow

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SIMULATION OF A BIOREACTOR Tiffany Tarrant Todd Giorgio

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  1. SIMULATION OF A BIOREACTORTiffany TarrantTodd Giorgio

  2. What is a Bioreactor? • Experimental device used to culture cells • Provides nutrient media, oxygen support, fluid environment, area to grow • Used both in laboratories and in industry-- specifically used in the lab portion of the BME 281: Biotechnology class

  3. BME 281: Biotechnology • Course goal: to integrate cellular and molecular biology with process bioengineering to describe the manufacture of products derived from mammalian cells

  4. Why Simulate? • based on initial lab results • quicker, more efficient, and less expensive

  5. Laboratory 22 days to prepare cells for bioreactor 5 days to obtain a significant amount of growth TOTAL: 27 days Simulation approximately 1 minute to enter experimental data and get results TOTAL: 1 minute Experimental Time Comparison

  6. Typical Cell Culture

  7. Cell Types • HeLa--common in research labs • ECV304--endothelial cells • 293--used in BME 282 lab • can be distinguished based on specific growth constants & the extent to which they are affected by local environmental limitations

  8. Past Work • Modeled simple exponential growth based only on cell-specific growth constant • Accounted for oxygen delivery limitation • Introduced different impeller types

  9. Simple Exponential Growth • unlimited growth • cell types distinguished based on k

  10. Oxygen Limitation Effects

  11. Impeller • different types influencing the amount of power that is delivered to the bioreactor system • increases oxygen dispersal throughout the system, thereby increasing delivery • forces imposed on cells due to stirring causes mechanical damage and cell death

  12. Impeller Types Rushton turbine Marine Propeller Paddle Anchor Helical ribbon

  13. Current Work • Incorporation of impeller effects on growth • Integration of ISF to balance oxygen delivery capabilities with cell death due to mechanical damage • Validation of model with actual lab results • Literature search to investigate other cell culture models

  14. Impeller Effects

  15. Integrated Shear Factor • Cell growth under different shear conditions can be correlated to an ISF factor

  16. Stirring Speed Effects via ISF • ISF related to speed of impeller and its distance from the walls of the bioreactor

  17. Experimental Comparisons • Given a time lag, model correlates with BME 282 data

  18. Other Models • No other model attempted to integrate several interrelated factors that affect cell growth • Instead, focused on one parameter or determining event • None incorporated oxygen delivery limitations

  19. Program Flowchart

  20. Parameter Effects on Growth

  21. Future Work • 1. Slight alterations to the program to make it more user-friendly • 2. Specific documentation of program procedure and functions

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