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Dr. Myrto - Panagiota Zacharof Supervisor: Dr.R.W.Lovitt

The Production Of Bacteriocins From Lactic Acid Bacteria. Dr. Myrto - Panagiota Zacharof Supervisor: Dr.R.W.Lovitt. LactoFermentation. Lactoproducts. Bacteriocins :Lantabiotics. Bacteriocins :Other Lantabiotics ?. Nisin. ?. ?. ?. ?. ?. ?. Bacteriocins :Reseach on other strains.

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Dr. Myrto - Panagiota Zacharof Supervisor: Dr.R.W.Lovitt

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  1. The Production Of Bacteriocins From Lactic Acid Bacteria Dr. Myrto-PanagiotaZacharof Supervisor: Dr.R.W.Lovitt

  2. LactoFermentation

  3. Lactoproducts

  4. Bacteriocins :Lantabiotics

  5. Bacteriocins :Other Lantabiotics ? Nisin ? ? ? ? ? ?

  6. Bacteriocins :Reseach on other strains L.plantarum 3 L.casei L.lactis

  7. Practical Steps Taken • Media Optimisation • Extraction strategy

  8. Practical Steps Taken • Chemical nature Protein assay trypsination Sizing

  9. Practical Steps Taken • Quantification Assay

  10. Practical Steps Taken • Concentration &Purification Method

  11. Practical Steps Taken • Self Life

  12. Results The 3 strains are indeed producing bacteriocins

  13. Results • Chemical Nature

  14. Results • Bacteriocins Activity Target strains Nisin & Bacteriocins

  15. Results • Bacteriocins Potency Target Strain Bacteriocins

  16. Results • Self Life

  17. The Finishing Line

  18. The Finishing Line • Further Developments

  19. Acknowledgments Dr.R.W.Lovitt Dr.M.Jones Dr.P.Williams Dr.I.Jung

  20. Thankyou!! • Any questions? • Email:M.Zacharof@swansea.ac.uk

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