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In silico feedback for in vivo regulation of a gene expression circuit. Shared by lxshen. Biological feedback. Signals Biological control circuits Implement of control. Comparison of control circuits. Synthetic biological circuits. Electronic circuits. Prosperous Easy to control
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In silico feedback for in vivo regulation of a gene expression circuit Shared by lxshen
Biological feedback • Signals • Biological control circuits • Implement of control
Comparison of control circuits Synthetic biological circuits Electronic circuits Prosperous Easy to control More functions Fast • Nature circuits robust • Difficulty in artificial circuits • Not easy to control • slow
model • Fourth-order linear ordinary differential equation • Identification experiment to determine five parameters fluorescence delay (fluorescence ≠ gene expression)
Applying model Ⅰ • Task: increasing fluorescence 4 or 7 times over a 7-h period • Open-loop(OL)control • Fail to regulate fluorescence to desired levels due to inaccuracies of the model and something unpredictable
Applying model Ⅱ • Closed-loop(CL) feedback control • Kalman filter
Significance • More predictable and accurate • Avoid the disadvantage of bio-circuits • Facilitate synthetic systems