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Putative binding protein. (%). count. ZNF313 (NM_018683). 29.8. 36. DAZAP2 (NM_014764). 2.5. 3. Yeast two-hybrid assay. eEF1A1 (NM_001402). 2.5. 3. HRG (NM_000412). 2.5. 3. AHSG (NM_001622). 1.7. 2. pGBKT7-XAF1. ZNF313 30% (36/121). CD14 (NM_001040021). 1.7. 2. pGBKT7.
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Putative binding protein (%) count ZNF313 (NM_018683) 29.8 36 DAZAP2 (NM_014764) 2.5 3 Yeast two-hybrid assay eEF1A1 (NM_001402) 2.5 3 HRG (NM_000412) 2.5 3 AHSG (NM_001622) 1.7 2 pGBKT7-XAF1 ZNF313 30% (36/121) CD14 (NM_001040021) 1.7 2 pGBKT7 CLU (NM_203339) 1.7 2 FHR (BC074957) 1.7 2 Representative ZNF313 clone PCOLCE (BC033205) 1.7 2 RPL36a (NM_001001) 1.7 2 Sel P (BC040075) 1.7 2 Others 51.2 62 Total 100.0 121 siControl siZNF313-1 siZNF313-2 siZNF313-3 H. sapiens RNF125 ZNF313 XAF1 Tubulin M. musculus ZNF313 T98G X. laevis ZFP313 R. norvegicus RNF114 H. sapiens ZNF313 B. taurus RNF114 P. troglodytes RNF114 C. familiaris RNF114 U2OS/XAF1 T98G G. gallus RNF114 0 1 2 3 0 1 2 3 ZNF313-V5 (g) ZNF313 (V5) XAF1 Tubulin H. sapiens RNF138 D. rerio RNF114 CHX (10 M) treatment for 24 h S. salar Zn313 1 Human 2 Chimp 3 Cattle 4 Dog 5 Rat 6 Mouse 7 Frog 8 Chicken 9 Salmon 10 Zebra fish H. sapiens RNF166 1. H. sapiens ZNF313(NP_061153.1) 99 93 94 89 90 87 72 57 53 Protein identity (%) 2. P. troglodytes RNF114(NP_001120978.1) 99 92 94 89 89 87 73 57 53 3. B. taurus RNF114(NP_001019702.1) 95 94 94 89 89 87 70 58 54 4. C. familiarisRNF114(XP_534453.3) 96 96 96 90 90 87 73 53 53 1.0 0.8 0.6 0.4 0.2 0 T98G 5. R. norvegicus RNF114(NP_001001517.1) 93 93 94 96 97 93 71 55 52 siControl siZNF313-1 6. M. musculusZFP313(NP_109668.2) 93 93 95 95 99 96 71 55 53 7. X. laevis ZFP313(AAT28738.1) 92 91 93 83 97 98 70 52 52 Relative XAF1 level 8. G. gallus RNF114(NP_001001767.2) 80 80 79 82 79 79 80 58 54 9. S. salar Zn313(NP_001133365.1) 72 72 72 67 69 69 68 71 67 10. D. rerio RNF114(NP_001001828.2) 71 71 71 71 71 71 70 70 79 0 12 24 36 48 (h) Protein homology (%) Hours after CHX addition Supplementary Fig. S1 b a c e f d g