### Cardiovascular Histology: Identifying Vessels & Tissue Layers ####

1. 243 Unit 3Labs 1 and 4

2. Histology Slides for the Cardiovascular System • Slides are presented in order of magnification • As you view the following slides, make sure you can accomplish these goals: • Can you identify the vessels and tissue layers observable on the slides? • Can you identify the specific structures or layers indicated by the numbered arrows or brackets? • At the end of a sequence, you will find the answers to the above for each vessel and layer

3. 4 4 1 2 3 3 Overview (40X), Masson stain for connective tissue

4. Overview (100X), Masson stain for connective tissue 4 3 6 9 7 10 5 8 1 2 3

5. 13 10 9 12 11 8 Vein or Artery? 400X

6. Vein or Artery? 400X 13 6 5 7 5 14

7. Artery, Vein, Nerve Tissue stained with Masson stain Slides 2 through 5: • Artery • Vein • Nerve fascicles • Skeletal Muscle Tissue • Lumen of the vein (with RBC’s) • Vein Tunica Media- smooth muscle layer • Vein Tunica Externa- connective tissue layer • Lumen of the Artery (with RBC’s) • Artery Tunica Externa- connective tissue layer • Artery Tunica Media- smooth muscle layer

8. Artery, Vein, Nerve Tissue stained with Masson stain Slides 2 through 5: 11. Artery Tunica Interna- endothelial cells (simple squamous) • Artery Tunica Interna- Internal elastic lamina • Capillary Tunica Interna- endothelial cells • Vein Tunica Interna- endothelial cells (simple squamous)

9. 3 4 3 2 2 2 1 Overview (40X), stained for elastic fibers

10. Vein or Artery? 400X 16 12 10 8 2 15 9

11. Vein or Artery? 400X 10 8 15 9 12

12. Vein or Artery? 400X 16 3 7 5 6 1

13. Vein or Artery? 400X 16 14 6 7 5

14. Artery, Vein, Nerve Tissue stained for Elastic Fibers Slides 8 through 12: • Artery • Vein • Nerve fascicles • Skeletal Muscle Tissue • Lumen of the vein • Vein Tunica Media- smooth muscle layer • Vein Tunica Externa- connective tissue layer • Lumen of the Artery (with RBC’s) • Artery Tunica Externa- connective tissue layer (demonstrating elastic fibers) 10. Artery Tunica Media- smooth muscle layer

15. Artery, Vein, Nerve Tissue stained for Elastic Fibers Slides 8 through 12: 11. Artery Tunica Interna- endothelial cells (simple squamous) • Artery Tunica Interna- internal elastic lamina • Capillary Tunica Interna- endothelial cells • Vein Tunica Interna- endothelial cells (dark band is due to the elastic fibers in the basement membrane) • Artery Tunica Media- external elastic lamina • Adipose cells

16. Vein or Artery? 400X 16 7 6 5 14 8

17. Vein or Artery? 100X 2 16 8 9 10 12 3 3

18. 8 11 12 10 9 Vein or Artery? 400X

19. Artery, Vein, Nerve Tissue, Nonspecific Stain Slides 15 through 16: • Artery • Vein • Nerve fascicles • Skeletal Muscle Tissue • Lumen of the vein • Vein Tunica Media- smooth muscle layer • Vein Tunica Externa- connective tissue layer • Lumen of the Artery (with RBC’s) • Artery Tunica Externa- connective tissue layer (demonstrating elastic fibers) 10. Artery Tunica Media- smooth muscle layer

20. Artery, Vein, Nerve Tissue, Nonspecific Stain Slides 15 through 16: 11. Artery Tunica Interna- endothelial cells (simple squamous) • Artery Tunica Interna- internal elastic lamina • Capillary Tunica Interna- endothelial cells • Vein Tunica Interna- endothelial cells (simple squamous) • Artery Tunica Media- external elastic lamina • Adipose cells

21. Specific Kind of Tissue? 400X 4 3 1 2 2 2

22. Cardiac Muscle Tissue Slides 20: • Nucleus of a Cardiac Muscle Cell • Intercalated Disc • Branching Fibers • Capillary Tunica Interna- endothelial cells

23. Identify Blood cells 3 2 1 2

24. Identify Blood cells 4 3

25. Identify Blood cells 2 4 3

26. Identify Blood cells 3 6 3 3

27. 7 3 5 5 Identify Blood cells

28. Identify Blood cells 7 2 3 3 3

29. Blood Cells Slides 22-27 • Basophil • Small Lymphocyte • Neutrophil • Eosinophil • Monoctye • Large Lymphocyte • Platelets