Alchemist II: High-Throughput Crystallization Screen System

1. AlchemistTM II: Capacity, reproducibility and efficiency of an high-throughput crystallization screen preparation system. Craig Sterling Director of Business Development Rigaku

2. Integration ModuleIncluding Robotic Arm, plate and block storage, mixing station, piercing station, and foil and tape sealing Automated Protein Crystallization Platform Phoenix RE Experimental Plate Setup Minstrel HT & Gallery700Incubators Imaging and Incubation AlchemistTM IIScreen Maker CrystalTrakTM Information Flow & Management

3. CrystalMationTM Features • AlchemistTM II, Phoenix RE, MinstrelTM HT imaging system and GalleryTM 700 incubators operate independently or as part of the completely integrated CrystalMation system • Allows instruments to be added over time to meet budgets • Easy and seamless integration • CrystalTrakTM relational database application • Easy-to-use design is manageable by tech level personnel • Mixing, Piercing & Sealing accessories for CrystalMationTM system • Low cost operation of $10 per plate including maintenance costs and chemical costs

4. CrystalTrakTM CrystalTrak seamlessly integrates the Rigaku Crystallization instruments, offering labs a complete end-to-end solution Relational database tracks all of the necessary information used in crystallization trials such as chemicals, screens, protein samples, plate type information, images, and annotations CrystalTrak simplifies and aids in the crystallization process by providing tools that enable users to greatly improve crystallization trial productivity with less user intervention. One-click optimization tools Intuitive screen design tools CrystalTrak Web offers remote access to data

5. Custom Screen Maker – The Alchemist II • The best accuracy in the industry for handling stock solutions of all viscosities, dispensing volumes as low as 1µl using the TapperTM technology • Produce liquid formulations without wasting chemicals during every cleaning, priming and wash cycle • 74 Barcoded easy to swap reagents • No tedious setup, cleaning and maintenance • Dedicated syringe for each reagent • Easy screen design with CrystalTrak

6. NEW Seal-tight BirdFeedersTM • New threaded cap, Seal-tight design • Long-term storage of stock solutions on deck • Works with standard stock solution bottles • Barcode Tracking of birdfeeders and stock solutions • Autoclave-able design • Easy-pour design for replacement of stocks • Four different stock solution sizes • 20ml in Birdfeeder with cap • Good for expensive stocks and additives • 125ml Nalgene bottles • 250ml Nalgene bottles • Three Syringe sizes (Eppendorf) • 100ul • 1ml • 10ml

10. Alchemist II

11. Integration ModuleIncluding Robotic Arm, plate and block storage, mixing station, piercing station, and foil and tape sealing Automated Protein Crystallization Platform Phoenix RE Experimental Plate Setup Minstrel HT & Gallery700Incubators Imaging and Incubation AlchemistTM IIScreen Maker CrystalTrakTM Information Flow & Management