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First Annual Meeting

First Annual Meeting. Pioneering the Use of Genomic Biomarkers in Assessing Risk to Environmental and Human Health. An established and effective collaboration A well-established need Particular local strengths: model systems high throughput approaches toxicology Strategy and goals.

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First Annual Meeting

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  1. First Annual Meeting

  2. Pioneering the Use of Genomic Biomarkers in Assessing Risk to Environmental and Human Health • An established and effective collaboration • A well-established need • Particular local strengths: • model systems • high throughput approaches • toxicology • Strategy and goals

  3. The Indiana University Consortium for Environmental Genomics and Toxicology • The Center for Genomics and Bioinformatics (IUB) • The Center for Environmental Health (IUSOM) • The School of Public and Environmental Affairs • The Center for Research in Environmental Science (IUB)

  4. Control mRNA RT RT Control Treatment Treated mRNA Control labeled target Microarray response data Treaated labeled target High-throughput experiments catalog all the genetic responses to drugs:

  5. The summary is a signature of a drug’s effects. • A gene expression signature of androgen stimulation was defined from gene expression profiles of LNCaP cells stimulated with the synthetic androgen R1881 for 12 hr and 24 hr, as compared to androgen-deprived LNCaP cells. • The 27 gene signature contains both androgen-induced and androgen-repressed genes, shown here by row-normalized heat map.

  6. Control mRNA RT RT Control Environmental stress Stress mRNA Control labeled target Gene response data Stressed labeled target A Strategy: High throughput water quality testing using gene expression signatures in Daphnia • Test stressors in the lab • Make a catalog of signatures • Use those signatures to guide toxicological work in mammals • Look for those signatures to assess water quality

  7. The Results: • Assessment – Sensitivity and information-rich measurement of "bio-active" titers. • Diagnosis – Enhanced understanding of the mechanisms of toxicity and a better assessment of risk. • Combining the evaluation of human and ecological risks. • The Goal: New regulatory standards that take advantage of these inexpensive, information-rich approaches.

  8. The Indiana University Consortium for Environmental Genomics and Toxicology

  9. First Annual Meeting

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