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Phosphate Levels and Macro Invertebrate Diversity. By: Samantha Cooney and Joshua McKain. Justification. Water quality differences are known to affect macro invertebrate diversity Phosphate levels are known to spike in Fernan Lake
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Phosphate Levels and Macro Invertebrate Diversity By: Samantha Cooney and Joshua McKain
Justification • Water quality differences are known to affect macro invertebrate diversity • Phosphate levels are known to spike in Fernan Lake • Could disequilibrium between phosphorus and nitrogen in the lake affect macro invertebrate diversity in lake outlet?
Introduction • Initial Question • Do high phosphate concentrations affect macro invertebrate biodiversity in riffles? • Hypothesis • If phosphate levels are high, macro invertebrate biodiversity will be low.
Methods • Disturb a .5 square meter area riffle with shovel • Collect macro inverts with a kick net • Organize inverts based on high quality, medium quality, and low quality species • Collect pH, DO, water temp., air temp, and phosphorous concentration at invert collection sites • Draw correlations using Anova
Materials • Shovel • Kick net • 4 sample tubes • Lab quest • Probes: DO, pH, temp • Bug viewer • Hach kit • Forceps • Dichotomous keys
Site 1 and 2 Site 3 Site 4 and 5
Site 4 had low diversity and pop. density because there was no water flowing the day before we collected our samples.
Analysis of the Variance to find if the means are correlated • (Ho: µ1=µ2) • (Ha: µ1≠µ2). • f* is not in the critical region, there for we accept (Ho: µ1=µ2) and reject the null hypothesis (Ha) that the means are not related. • P-value 0.657637> 0.05 therefore we accept Ho
Application in the classroom • Mirror Adventure Learning • Place-based learning and field trips • More time collecting data on inverts • Lessons on dichotomous keys • Blogging • AL vs. DL
Conclusion • Data supported hypothesis • Phosphate concentrations seem to have an affect on macro invertebrate diversity • Difference of means are related • Too little data may have skewed results • More time in data collection • More time identifying individual species • Confusion with units for phosphate