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Events at initiation of transcription

Events at initiation of transcription. Let R = RNA polymerase, P = promoter (closed), and Po= promoter (open). ATP + UTP*. k f. K B. RP. RPo. R + P. k r. Abortive transcripts. ApUp*U. [ApUp*U]. lag. time. Abortive initiation assay. 1. 1. 1. 1. 1. 1. K B k f. K B k f. [R].

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Events at initiation of transcription

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  1. Events at initiation of transcription

  2. Let R = RNA polymerase, P = promoter (closed), and Po= promoter (open) ATP + UTP* kf KB RP RPo R + P kr Abortive transcripts ApUp*U [ApUp*U] lag time Abortive initiation assay

  3. 1 1 1 1 1 1 KB kf KB kf [R] [R] kf kf Measure kf and KB from lag time vs. 1/[R] Lag time in abortive initiation assay is inversely proportional to [R]. Lag time = x + Lag time Slope = Y-intercept =

  4. Effect of wild-type and pc mutantλ repressors on activity of PR & PRM

  5. Effect of Operator Mutations on Transcriptional Control of PR&PRM OR1+OR2-OR3+ OR1-OR2+OR3-

  6. Effect of λ-pc mutations on KB and k2

  7. Architecture of λOR

  8. Mutations in the Activating Region ofλ Repressor Glu Glu Glu

  9. Mutations in the δ subunit of RNA polymerase that interfere w/λ repressor-mediated activation of PRM transcription

  10. Effect of mutations in the δ subunit of RNA polymerase on activator-dependent and independent transcription of the lac promoter

  11. A model for interaction of the δ subunit of RNA polymerase with λ repressor

  12. The Awesome Power of Genetics

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