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Project has supported a graduate bioengineering student.

Materials World Network: Dynamic Materials with Triggerable Adhesion Motifs Andrés J. García , Georgia Tech Research Corporation, DMR 0909002.

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Project has supported a graduate bioengineering student.

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  1. Materials World Network: Dynamic Materials with Triggerable Adhesion MotifsAndrés J. García, Georgia Tech Research Corporation, DMR 0909002 • We have engineered hydrogels that present a caged-RGD peptide. RGD is a potent cell adhesive peptide. The DNMPB cap on the peptide is cleaved by 351nm light. This research provides a platform to study the effects of spatiotemporal control of adhesion in culture and in the body. • We have shown in an in vivo inflammation model that delayed activation of caged-RGD results in fibrous capsule attenuation. • We have demonstrated that temporal activation of caged-RGD modulates myoblast differentiation. • We have shown that caged-RGD can be used to study protein localization and cell contractile force to nascent cell adhesions. In vivo activation of caged RGD peptide modulates inflammatory responses. (A) Fibrous capsule thickness of hydrogel implants after one week. (B) Fibrous capsule thickness of Day 0 exposed caged RGD compared to controls (C) Capsule thickness for hydrogels presenting caged RGD peptides activated at different time points. (D) Histological sections of implanted hydrogels with Mason’s tri-chrome stain demonstrating the resulting inflammatory fibrous capsule.

  2. Materials World Network: Dynamic Materials with Triggerable Adhesion MotifsAndrés J. García, Georgia Tech Research Corporation, DMR 0909002 • Project has supported a graduate bioengineering student. • Graduate student and PI visited collaborator at the Max Planck Institute for Polymers in Germany to conduct experiments as part of MWN award. Another trip is planned for this year. • We hosted an under-represented minority undergraduate student for a summer project. Publications Wirkner M, Alonso JM, Maus V, Salierno M, Lee TT, García AJ, Del Campo A. Triggered cell release from materials using bioadhesive photocleavable linkers. Advanced Materials 2011. doi: 10.1002/adma.201100925. Focal adhesion localization and force increase following caged-RGD activation on mPADs. (A) left, MEF cell spread on mPADs substrate showing UV irradiated post. Dotted yellow line denotes cell outline; right, cell contractile force on single post vs. time. (B) left, DiI labeled post tracked at 0 and 600 seconds; focal adhesion intensity at 0 and 600 seconds. Plot of focal adhesion intensity vs. time including controls.

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