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Initial results from the screening of PROSOPE and Roscoff cultures

Initial results from the screening of PROSOPE and Roscoff cultures. Florence LE GALL, Héloïse FELMAN, Frédéric PARTENSKY, Nathalie SIMON, Dominique MARIE, Daniel VAULOT. Surface water of ASTAN. 2 filtrations on 3µm. Culture in liquid medium K/10, K/100, K/10+Si,F/20. Culture on plate

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Initial results from the screening of PROSOPE and Roscoff cultures

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  1. Initial results from the screening of PROSOPE and Roscoff cultures Florence LE GALL, Héloïse FELMAN, Frédéric PARTENSKY, Nathalie SIMON, Dominique MARIE, Daniel VAULOT

  2. Surface water of ASTAN 2 filtrations on 3µm Culture in liquid medium K/10, K/100, K/10+Si,F/20 Culture on plate K medium (0,6%agar) Serial dilutions K/10 The first steps of isolation Enumeration by flow cytometry

  3. Serial dilutions Flow cytometry sorting • 24 eukaryotes • 14 Synechococcus 38 strains isolated Isolation and purification of microalgae 160 precultures (PROSOPE)

  4. PROSOPE 157-13 PROSOPE 53-4 PUB PEB PEB PUB wavelength wavelength Synechococcus spectra

  5. Neo Viola HPLC analysis and presence of caracteristic pigments • PROSOPE culture 3 Prasinophyceae Chl a Chl b MgDVP Pras MgDVP : Mg-2,4-divinyl pheoporphyrin a5 Pras : Prasinoxanthin Diadchr : Diadinochrome Neo : Neoxanthin Viola : Violaxanthin Chl b : Chlorophyll b Chl a : Chlorophyll a Lut : Lutein ß-car : ß-carotene Diadchr • PROSOPE culture 18 Chlorophyceae Chl a Chl b Lut Neo Viola ß-car

  6. PROSOPE culture 96 Prymnesiophyceae Chl c2 19’HF Chl a Fuco Diadino Chl c3 19’HF :19’-Hexanoyloxyfucoxanthin Fuco : Fucoxanthin Diadino : Diadinoxanthin Chl c2 : Chlorophyll c2 Chl c3 : Chlorophyll c3 Chl a : Chlorophyll a Diato : Diatoxanthin ß-car : ß-carotene • ASTAN culture R 000412-4 Bacillariophyceae Chl a Fuco Chl c2 Diadino Chl c3 Diato ß-car

  7. Optical microscopy Transmission electronic microscopy Morphological characterization

  8. Euk Astan (K) PRO_90 PRO_96 PRO_18-1 PRO_1 PRO_114-2 PRO_118-1 PRO_140-3 PRO_36 PRO_54 Euk Astan (F/2) PRO_63 PRO_64 PRO_32 Peuk Astan PRO_5 PRO_3 Prymnesiophyceae Bolidophyceae Prasinophyceae PRO_22 Chlorophyceae 1000 Cultures from - Med Sea (PRO)- Roscoff E. huxleyi Iso. Galb. 100 Phaeocystis Micro pusi Ostreo. taur Chl fluorescence Ostreo. ocea 10 Prymnesiophyceae Bolid.pacifi Bolid.med Diatom 1 0.1 0.1 1.0 10.0 Scatter

  9. Summary Prymnesiophyceae : 11 strains Prasinophyceae : 3 strains Chlorophyceae : 1 strain Bacillariophyceae : 1 strain

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