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Lumbar Punctures

Lumbar Punctures. Rachicentesis. The history. 1889- First procedure to access the dural space consisted of a cut down procedure to help decrease intracranial pressure in people in TB meningitis by Walter Essex Wynter

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Lumbar Punctures

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  1. Lumbar Punctures Rachicentesis

  2. The history • 1889- First procedure to access the dural space consisted of a cut down procedure to help decrease intracranial pressure in people in TB meningitis by Walter Essex Wynter • 1891- The first needle was used to access the dural space by HeinricheQuincke • 1893- Arthur H. Wentworth was the first to perform the procedure at Harvard, interestingly though he was almost tossed in jail by anti-vivisectionists for his experimentation, but was acquitted • 1898- Karl August Bier and his assistant August Hildebrandt attempted first spinal anesthetic by doing lumbar punctures on each other with injection cocaine into the intrathecal spaces

  3. Anatomy 4 5

  4. Lumbar Puncture needles of all sizes

  5. The Procedure • Palpate the posterior superior iliac crest bilaterally, and move medially to the spine and find the L3-L4 intervertebral space with the patient in either fetal position in a lateral recumbent position or hunched over in a sitting position as determined by your needs • Palpate the L2-L3 and L4-L5 spaces above and below, mark the appropriate space that you want to enter, preferably with the cap of a pen or something that causes an indentation that won’t be erased during sterilization • Open the kit, and put on sterile and prep the kit, assembling the opening pressure gauge and opening the bottles and having them lined up and numbered, and having the lidocaine drawn up • Prep the area with betadine, avoid chloroprep as this can cause an aseptic meningitis, then drape the area to create a sterile field

  6. Procedure Continued…. • Anesthetize the area using initially a 25 gauge needle to raise a wheal, and then a 20 gauge needle to hit deeper structure create a wide arc during anesthetizing, in case another level will need to be done, the needle should aspirated each time prior to administration to ensure no blood is present (The lumbar puncture needle itself can be used for deeper structures • Insert the needle in a cephalic direction towards the umbilicus, orient the bevel parallel to the longitudinal dural fibers, to decrease the chance of you cutting them (** in a lateral recumbent position this should mean the bevel is facing up, in a sitting position, means the bevel should be facing sideways ) • Advance the needle with occasionally removing the stylet to ensure no fluid return, in normal adults the needle will have to inserted 4-5cm before getting close to the dural space, if no fluid is returned replace the stylet and either advance or withdraw the needle and recheck • if bone is struck at shallow depth, redirect needle more cephalad; • if at deeper depth – more caudally

  7. The Procedure (Continued….) • After fluid return if the patient is a lateral recumbent position a manometer can be attached for opening pressure reading (** the patient’s legs should be straightened prior to checking opening pressure) • Collect around 3-4mL in each tube, if the flow is slow you can ask the patient to Valsalva, rotate the needle 90 degrees to face cephalad, or have an assistant press on the abdomen • Replace the stylet and remove the needle at the end, clean off the area, and place a sterile dressing, and have the patient lay in a supine position for 1-2 hours.

  8. Indications for Lumbar puncture • Ruling out and/or determining the cause of meningitis, subarachnoid hemorrhage, carcinamatous meningitis, inflammatory diseases • Determining an opening pressure e.gpseudotumourcerebri • Therapeutic intervention to reduce pressure for pseudotumourcerebri, TB meningitis, cryptococcal meningitis etc. • Intrathecal administration of drugs (malignancy), contrast (myelogram), or radioisotopes (CSF leak) • Consideration for spinal drain insertion

  9. ContraindicationS • Elevated Intracranial pressure- head trauma, Intracranial mass (especially in the posterior fossa) • Cutaneous infection overlying the site • Noncommunicating hydrocephalus • Coagulopathy (thrombocytopenia is relative contraindication though no trials show increased risk of bleeding)

  10. Complications • Uncalhernation • Epidural/perivertebral abscess • Paresthesias- temporary or permanent • Post lumbar puncture headache • Spinal or epidural bleeding (with some case reports of perforation of AVM’s) • Adhesive arachnoiditis • Late onset of epidermoidtumours to thecal sac • Back pain

  11. Opening pressure measurements • Making adjustments for opening pressures for sitting or in the flexed position in lateral decubitus position has been attempted but most of these trials are small and in children • 1 small trial tried finding an adjustment for sitting versus lateral decubitus but this has not been reproduced in larger trials • General consensus at this time for opening pressure measurements is… • Opening pressure is preferably taken in a lateral decubitus position with the legs extended and head not flexed with the patient comfortable • if anatomy precludes LP in lateral decubitus but opening pressure is critical: spinal needle inserted in sitting position → needle withdrawn just out of thecal sac → patient placed in relaxed, lateral decubitus position → needle readvanced slightly into thecal sac. • If opening pressure is > 35cm H20 then the needle should be removed immediately the CSF from the manometer should be used for analysis and no further amount should be drawn • At opening pressures close to 35cm H20 the pressure should not be decreased by > 50% to avoid risk of herniation

  12. Indications for CT • Addressed in 2001 trial at Yale-New Haven1. Trial enrolled 301 people, 78% of whom underwent CT prior to lumbar puncture • Data was gathered about clinical features, socioeconomic background, and immunocomprimising state • NIH stroke scale was used to monitor neurological function involving level or alertness, answering questions, and neurological exam. • CT scan was obtained and was read by 2 neuroradiologist who did not know the clinical history. • The scan was read as normal, focal abnl +/- mass effect, or nonfocalabnl +/- mass effect • Only 3 scans had a disagreement between the 2 neuroradiologists, at which time a 3rd radiologist was brought in • High risk features on CT included midline shift, loss of suprachiasmatic and basilar cisterns, posterior fossa mass, loss of the superior cerebellar cistern, loss of the quadrigeminal plate cistern

  13. Indications for CT • Of the 234 that underwent CT 56 were found to have abnormal findings, with only 4 showing mass effect that prompted the clinician to avoid doing LP, 2 of whom died from herniation (29 had focal lesion without mass effect, 12 nonfocal lesion without mass effect, 9 focal lesion with mass effect [3 of whom had severe mass effect], 2 nonfocal lesions with mild mass effect, 4 combination of focal and nonfocal lesions with no mass effect) • Nonfocalabnl were hydrocephalus, meningeal enhancement, or subarrachnoid hemorrhage • Focal abnormalities were mass lesion, stroke, or disease or periventricular white matter • Remaining 52 with abnormal results and underwent LP had 0 herniations 1 week out • Factors identified with highest risk ratio were age >60, immunocomprimised, h/o CNS disease, seizure in the last week, alertness, answering questions, following commands, and abnlneuro exam • Mean time of admission from patients undergoing CT versus not was longer at 5.3hr versus 3 hours with P<.001 • Trend was longer of initiation of antibiotics with patients that had to wait for CT • Patient with no significant clinical features had negative predictive value on head CT of 97%, of the three that were misdiagnosed only 1 had mild midline shift, but all 3 were tapped with no complications

  14. Lumbar puncture for subarachnoid hemorrhage • Within 12 hours of bleed 3 retrospective trials in the 90’s showed 100% sensitivity with CT scan, and 93% sensitivity after 12 hours • CT needs to be a thin cut CT which is properly aligned with the hard palate • CT is limited by motion artifact, blood becomes more isodense at Hgb <10, blood and bone become hard to differentiate in small hemorrhage, and time from onset of symptoms allowing dynamics of cerebrospinal fluid and spontaneous lysis can result in the rapid clearing of subarachnoid blood • Literature is very sketchy on head to head trials and most is consensus agreement • LP per current guidelines are recommended in patient’s with negative, equivocal, or technically inadequate head CT’s when clinical suspicion is very high • Furthermore a negative head CT and negative lumbar puncture • Furthermore there is no clear consensus on if xanthochromia or non clearing bloody tap/erythrophagocytosis is better for determining presence of subarrachnoid hemorrhage , as the former is complicated by in vitro lysis of cells or too early to present the latter is complicated by traumatic tap • Consensus is that if either is present and clinical suspicion is high enough that a tap has either xanthochromia or non clearing blood a more dedicated vascular imaging • Atypical headache, severe/sudden headache, seizures, focal neurological sx’s, high risk history (smoker, etoh use, HTN, connective tissue dz, polycystic kidney dz, sickle cell, alpha 1 antitrypsin) • This is has further been validated by several trials in annals of emergency medicine in 2008 which found a sensitivity of 100%, specificity of 68%, PPR of 3.03, and NPR of 0 from a negative head CT and lumbar in excluding a diagnosis of subarachnoid hemorrhage

  15. CSF analysis • Gram stain: Sensitive in 60-80% of bacterial meningitis and 40-60% range in partially treated bacterial meningitis • Protein - can be adjusted to RBC by decreasing protein concentration by 1 mg/dLor .01g/L for every 1,000 RBC/mm3 but both levels have to be drawn from the same tube. • Is the most sensitive indicator of intracranial pathology but not very sensitive. Also, normal levels vary a lot from lab to lab. • Glucose- normally 60% of serum glucose, though in severe hyperglycemia this ratio breaks down as CSF glucose levels do not exceed 300mg/DL • Various PCR’s have a very high sensitivity and specificity ratio usually in the 95-100% range • Antibodies have large degree of variability associated with disease process • Oligoclonal bands: Nonsensitive indicator of inflammation • 14-3-3, tau >12,000 pg/mL, neuron-specific enolase >35ng/mL- CSF proteins for prion disease • Of note the sensitivity of prion disease in 1 large trial with >1000 patients was 94% with a specificity of 84%9

  16. CSF analysis continued • CSF LDH: Several trial have the shown the utility of CSF LDH in not only determining the prognosis of treatment • CSF levels > 35 has a high negative predictive value of ruling out aseptic meningitis and a 75% positive predictive value of encephalitis10 • CSF LDH can’t help determine between pyogenic and TB meningitis • Few trials looked at CSF LDH and reponse to chemotherapy or progression of leptomeningeal disease11 • CSF cryptococcal Ag: 94% sensitivity, titers can not be used to guide fungal therapy, higher initial titers have poor prognosis as expected

  17. TB meningitis per LP • Characteristic CSF findings are elevated WBC with lymphocyte predominance with elevated protein and low serum glucose, in the right clinical setting. • Difficult area as the gold standard has always been culture which has required four large taps of 15mL of CSF per tap with results needing 4-8 weeks with only sensitivity at 86% despite all this, this further increased if staining is only done on CSF sediment • TB PCR has shown some promise with a high specicity of 86%; however, continues to have a sensitivity of 50%, though recently developed PCR using several genes for amplification have brought this up to 85%, though these are currenlty in trials.7 • Negative CSF examination for acid-fast bacilli or M. tuberculosis DNA neither excludes the diagnosis of TBM nor obviates the need for empiric therapy if the clinical suspicion is high • CSF chloride was classically used as a marker for TB meningitis, where depressed values were considered pathognomonic. However, numerous trials of shown that it is more a reflection of serum chloride levels and depletion is more due to dehydration from frequent vomiting due to high ICP then any part of the actual disease process • CSF adenosine deaminase: Meta analysis showed that ADA can not help differentiate between TB meningitis and bacterial meningitis but a range in the values help increase the probability of the patient having TB meningitis. Ranges identified in the meta analysis include levels of 1-4U/L w/ sensitivity of > 93% and specificity of <80% in excluding TB. Values between 4-8 could not provide help. Values > 8 had a sensitivity of <59% and specificity of >96% in improving the diagnosis of TB.

  18. Neurosyphilis • Causes for tapping patient’s with neurosyphilis- High RPR titers, Signs or symptoms of neurosyphilis, immunocomprimised state, or congenital syphilis • The serum VDRL is positive in 72% of patients with primary syphilis, nearly 100% of patients with secondary syphilis, 73% of patients with latent syphilis, and 77% of patients with tertiary syphilis. • Estimates of a negative CSF VDRL in the setting of neurosyphilis are unknown but expected to be > 25%. • TreponemaAb test are unable to used because any minute contamination will cause false positive along with IgG passing through the blood brain barrier. • Current thought process, especially in setting of HIV is • Definite neurosyphilis: Positive serology FTA Abs, MHA-TP + CSF VDRL • Probable neurosyphilis: Positive serology – CSF VDRL with (CSF monos > 10 or protein >60) and neurological symptoms • Possible neurosyphilis: Positive serology – CSF VDRL with (CSF monos > 10 or protein >60) but no neurological symptoms

  19. References • Rodrigo Hasbun, M.D., James Abrahams, M.D., James Jekel, M.D., and Vincent J. Quagliarello, M.D. (12/13/01). Computed Tomography of the Head before Lumbar Puncture in Adults with Suspected Meningitis. N Engl J Med 2001; 345:1727-1733. A • Perry JJ, Spacek A, Forbes M, Wells GA, Mortensen M, Symington C, Fortin N, StiellIG. Is the combination of negative computed tomography result and negative lumbar puncture result sufficient to rule out subarachnoid hemorrhage? Ann Emerg Med. 2008 Jun;51(6):707-13. • Arendt , Katherine. (11/2009). AtraumaticLumbar Puncture Needles- After All These Years, Are We Still Missing the Point?. The Neurologist • Volume 15, Number 1, 17-20. • http://www.google.com/imgres?imgurl=http://doctorgrasshopper.files.wordpress.com/2010/01/lots-of-lumbar-puncturepics.jpg&imgrefurl=http://doctorgrasshopper.wordpress.com/tag/lumbarpuncture/&usg=__lwv_TtEXnWFDYMVkDFm28UnTPxc=&h=661&w=675&sz=152&hl=en&start=4&zoom=1&tbnid=XjHDImWj4KAZEM:&tbnh=135&tbnw=138&ei=8OHIULLnDqS0AGTvoGoDQ&prev=/search%3Fq%3Danatomy%2Bfor%2Blumbar%2Bpuncture%26um%3D1%26hl%3Den%26sa%3DN%26gbv%3D2%26tbm%3Disch&um=1&itbs=1 • http://www.google.com/imgres?imgurl=http://antranik.org/wp-content/uploads/2011/11/diagram-of-lumbar-puncture-spinal-tap.jpg&imgrefurl=http://antranik.org/central-nervous-system-spinal-cord/&usg=__ALiKM0V_R1_K8STzddVwmXanvT0=&h=519&w=580&sz=149&hl=en&start=13&zoom=1&tbnid=HOaJ84d0iCmbSM:&tbnh=120&tbnw=134&ei=8OHIULLnDqS-0AGTvoGoDQ&prev=/search%3Fq%3Danatomy%2Bfor%2Blumbar%2Bpuncture%26um%3D1%26hl%3Den%26sa%3DN%26gbv%3D2%26tbm%3Disch&um=1&itbs=1 • Elias J, De Coning JP, Vorster SA, Joubert HF. The rapid and sensitive diagnosis of tuberculous meningitis by the detection of tuberculostearic acid in cerebrospinal fluid using gas chromatography-mass spectrometry with selective ion monitoring. Clin Biochem. 1989 Dec;22(6):463-7. • Grace E. Marx 1 and Edward D. Chan. TuberculousMeningitis: Diagnosis and Treatment Overview. Tuberc Res Treat. 2011; 2011: 798764. • http://www.medmerits.com/index.php/article/neurosyphilis/P8, Neurosyphilis, 12/12/12 6:10PM. • I. Zerr, MD, M. Pocchiari, MD, S. Collins, MD. Analysis of EEG and CSF 14-3-3 proteins as aids to the diagnosis of Creutzfeldt–Jakobdisease. Neurology September 26, 2000 vol. 55 no. 6 811-815. • T. John*, C. Ittycheria, J. George, C. Jacob, A. Jacob, A. George. CSF LDH estimation to differentiate pyogenic and viral meningitis and its role in tuberculous meningitis.. http://registration.akm.ch/einsicht_iframe.php?XNABSTRACT_ID=121604&XNSPRACHE_ID=2&XNKONGRESS_ID=136&XNMASKEN_ID=900. • Twijnstra Albert, Zanten, Anton. Serial lumbar and ventricle CSF LDH activities in patients with leptomeningealdisesae from solid and haemotological disease. Journal of neurology 1987;50(3) 313-320. • Spinello Antinori*, Anna Radice and Laura Galimberti. The Role of Cryptococcal Antigen Assay in Diagnosis and Monitoring of Cryptococcal Meningitis . J. Clin. Microbiol. November 2005 vol. 43 no. 11 5828-5829

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