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DNA, Chromosomes and the Nucleus

DNA, Chromosomes and the Nucleus. DNA Structure. DNA Structure. What are some of the important structural features of the DNA double helix?. DNA Structure. In addition to B-DNA what other forms exist?. DNA Structure. What is supercoiled DNA and what effect does it have?

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DNA, Chromosomes and the Nucleus

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  1. DNA, Chromosomes and the Nucleus

  2. DNA Structure

  3. DNA Structure • What are some of the important structural features of the DNA double helix?

  4. DNA Structure • In addition to B-DNA what other forms exist?

  5. DNA Structure • What is supercoiled DNA and what effect does it have? • Can it occur in linear DNA molecules?

  6. DNA Structure • What is the difference between topoisomerases and DNA gyrase? • How can one bring about the denaturation and renaturation of DNA?

  7. DNA Structure

  8. DNA Structure • What is the DNA melting temperature?

  9. Genomic DNA • How do we define the term “genome”? • How is genomic size expressed? • Is there a relationship between genomic size and complexity of the organism or virus?

  10. Genomic DNA

  11. Genomic DNA • What are restriction enzymes and how have they helped us to study DNA?

  12. Genomic DNA • How can restriction fragments be characterized?

  13. Genomic DNA • How is restriction mapping carried out and what information does it provide?

  14. Genomic DNA • How is the chain termination method of Sanger used to determine the sequence of a DNA molecule?

  15. Genomic DNA • What is bioinformatics and proteomics? • How much variation is there in the human genome?

  16. Genomic DNA • How did the work of Britten and Kohne help explain why eukaryotic genomes are so large?

  17. Genomic DNA • What is the difference between tandemly repeated DNA versus interspersed repeated DNA?

  18. Genomic DNA • What are the possible functions of this tandemly repeated DNA? • What about the role of this DNA fingerprinting? • in certain genetic diseases?

  19. DNA Packaging • How is the bacterial genome structured to fit inside of the small cell? • What are bacterial plasmids?

  20. DNA Packaging • What is the relationship between DNA and chromosomes in eukaryotic cells? • What are nucleosomes?

  21. DNA Packaging • What experimental evidence supported the existence of nucleosomes?

  22. DNA Packaging • How is the nucleosome organized? Where is the H1 histone protein?

  23. DNA Packaging • How are nucleosomes packaged to form chromosomes? • What is the DNA packing ratio?

  24. DNA Packaging • How does mitocondrial and chloroplast DNA compare with nuclear DNA?

  25. Nucleus • What do we know about nuclear structure?

  26. Nucleus • What are the major features of the nuclear pore complex?

  27. Nucleus • What kinds of molecules are transported across the nuclear envelope?

  28. Nucleus • How are molecules transported across the nuclear envelope?

  29. Nucleus • What role does the nuclear matrix serve? • What role does the nuclear lamina serve? • What is the function of the nucleolus?

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