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Suppl. A. Vec ShcA ShcC. KU-YS cells. WB: a FLAG a hShcA a hShcC a -tubulin.
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Suppl. A Vec ShcA ShcC KU-YS cells WB: a FLAG ahShcA ahShcC a-tubulin Specific antibody against human ShcC protein. We raised a new anti-ShcC antibody against human ShcC : ahShcC, which shows no cross reactivity with human ShcA. To evaluate its specificity against human ShcC, either Flag-tagged ShcA or ShcC was expressed in KU-YS cells which is known to have no endogenous expression of ShcC. Results of western blot analysis using indicated antibodies are shown.